利用核糖体RNA、寡核苷酸和随机克隆的DNA探针进行沙眼衣原体的DNA指纹分析。

DNA fingerprinting of Chlamydia trachomatis by use of ribosomal RNA, oligonucleotide and randomly cloned DNA probes.

作者信息

Scieux C, Grimont F, Regnault B, Grimont P A

机构信息

Laboratoire de Bactériologie-Virologie, Hôpital St-Louis, Université Paris VII.

出版信息

Res Microbiol. 1992 Oct;143(8):755-65. doi: 10.1016/0923-2508(92)90103-u.

DOI:10.1016/0923-2508(92)90103-u

PMID:1298028

Abstract

DNA fingerprinting of 15 reference strains and 24 clinical isolates of Chlamydia trachomatis, 2 strains of C. psittaci and one strain of C. pneumoniae was studied by use of universal 16 + 23S RNA from Escherichia coli, 16S rDNA-directed oligonucleotide and randomly cloned chlamydial DNA probes. The rRNA-gene restriction patterns (ribotypes) enabled the differentiation of chlamydial species. Following DNA cleavage by restriction endonuclease PvuII, lymphogranuloma venereum and trachoma biovars of C. trachomatis could be differentiated. An oligonucleotide, designed to hybridize the C. trachomatis 16S rDNA, also allowed for both species-specific identification and biovar typing of C. trachomatis human strains. Molecular typing system using 3 lambda clones containing C. trachomatis serotype E random DNA inserts, combined to ribotyping, revealed 12 groups of variable banding patterns within C. trachomatis, and could provide an alternative epidemiological tool.

摘要

利用来自大肠杆菌的通用16 + 23S RNA、16S rDNA导向的寡核苷酸和随机克隆的衣原体DNA探针，对15株沙眼衣原体参考菌株、24株沙眼衣原体临床分离株、2株鹦鹉热衣原体菌株和1株肺炎衣原体菌株进行了DNA指纹分析。rRNA基因限制性图谱（核糖型）能够区分衣原体种类。在用限制性内切酶PvuII切割DNA后，可区分沙眼衣原体的性病淋巴肉芽肿和沙眼生物变种。一种设计用于与沙眼衣原体16S rDNA杂交的寡核苷酸，也可用于沙眼衣原体人源菌株的种特异性鉴定和生物变种分型。使用3个含有沙眼衣原体血清型E随机DNA插入片段的λ克隆并结合核糖分型的分子分型系统，揭示了沙眼衣原体内部12组可变的条带模式，并可提供一种替代的流行病学工具。