Peterson E M, de la Maza L M
Department of Pathology, University of California, Irvine 92717.
J Clin Microbiol. 1988 Apr;26(4):625-9. doi: 10.1128/jcm.26.4.625-629.1988.
DNA from a total of 60 Chlamydia trachomatis isolates was examined by restriction endonuclease analysis. Strains from all established biovars and serovars were tested. There was great diversity between the mouse biovar and the lymphogranuloma venereum (LGV) and trachoma biovars. The LGV and trachoma biovar isolates generated similar fragment patterns; however, distinct fragments appeared to be unique to both biovars, thus allowing differentiation of these two major groups. In most cases, strains of the same serovar could be differentiated from one another when a battery of restriction enzymes was used. In addition, in some cases, certain restriction fragments appeared to be characteristic of strains from a particular geographical location. The DNA patterns generated by all C. trachomatis isolates differed greatly from the DNA patterns generated from the Chlamydia psittaci isolates tested, including TWAR, a human C. psittaci strain.
运用限制性内切酶分析法对总共60株沙眼衣原体分离株的DNA进行了检测。对所有已确定的生物变种和血清型的菌株都进行了测试。小鼠生物变种与性病性淋巴肉芽肿（LGV）及沙眼生物变种之间存在很大差异。LGV和沙眼生物变种的分离株产生了相似的片段模式；然而，不同的片段似乎是这两个生物变种各自独有的，从而能够区分这两个主要类别。在大多数情况下，当使用一系列限制性酶时，同一血清型的菌株能够相互区分。此外，在某些情况下，某些限制性片段似乎是来自特定地理位置的菌株所特有的。所有沙眼衣原体分离株产生的DNA模式与所测试的鹦鹉热衣原体分离株产生的DNA模式有很大不同，其中包括人鹦鹉热衣原体菌株TWAR。
