School of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Polysaccharide Materials and Modifications, Guangxi Minzu University, Nanning, 530008, People's Republic of China.
College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, People's Republic of China.
Curr Microbiol. 2024 Feb 23;81(4):105. doi: 10.1007/s00284-023-03594-5.
A bacterium designated GXH0434 was isolated from sea shore samples collected from Weizhou Island, Beihai, Guangxi, China. The organism is motile, strictly aerobic, and possesses a rod-coccus cell cycle in association with the growth phase. It can grow at 15-45 °C (optimum 37 °C), at pH 6.0-11.0 (optimum 6.0), and at 0-20% (w/v) NaCl (optimum 5.0-8.0%). The strain is positive for peroxidase and oxidase activity, negative for Voges-Proskauer test, can hydrolyze Tween 20, Tween 60, Tween 80, casein, and is able to produce siderophore and has the function of nitrogen fixation. Molecular phylogenetic analysis based on 16S rRNA gene sequences indicated that GXH0434 was most closely related to Microbulbifer halophilus KCTC 12848 with the similarity of 97.2%, followed by Microbulbifer chitinilyticus JCM 16148 (97.1%) and Microbulbifer taiwanensis LMG 26125 (96.5%). The digital DNA-DNA hybridization and the average nucleotide identity values between GXH0434 and Microbulbifer halophilus KCTC 12848 were 28.90% and 83.38%, respectively, which were below thresholds of species delineation. The genomic DNA G+C content of the strain was 61.9%. The major fatty acids were iso-C, C, iso-C 3-OH, iso-C and Summed features 8 (C ω7c and/or C ω6c). The major polar lipids detected in GXH0434 were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC). The major respiratory quinone was ubiquinone Q-8. Based on the above polyphasic classification indicated strain GXH0434 represents a novel species of the genus Microbulbifer, for which the name Microbulbifer litoralis sp. nov. is proposed. The type strain is GXH0434 (= MCCC 1K07158 = KCTC 92169).
从中国广西北海涠洲岛采集的海岸样本中分离到一株被命名为 GXH0434 的细菌。该菌是运动的，严格需氧的，其杆状-球菌细胞周期与生长阶段相关。它可以在 15-45°C（最佳 37°C）、pH6.0-11.0（最佳 6.0）和 0-20%（w/v）NaCl（最佳 5.0-8.0%）下生长。该菌株过氧化物酶和氧化酶活性阳性，Voges-Proskauer 试验阴性，可水解吐温 20、吐温 60、吐温 80、酪蛋白，并能产生铁载体，具有固氮功能。基于 16S rRNA 基因序列的分子系统发育分析表明，GXH0434 与盐单胞菌属的嗜盐微杆菌 KCTC 12848 最为密切，相似度为 97.2%，其次是噬几丁质微杆菌 JCM 16148（97.1%）和台湾微杆菌 LMG 26125（96.5%）。菌株 GXH0434 与嗜盐微杆菌 KCTC 12848 之间的数字 DNA-DNA 杂交值和平均核苷酸同一性值分别为 28.90%和 83.38%，均低于种的界定阈值。该菌株的基因组 DNA G+C 含量为 61.9%。主要脂肪酸为 iso-C、C、iso-C 3-OH、iso-C 和 Summed features 8（C ω7c 和/或 C ω6c）。在 GXH0434 中检测到的主要极性脂是双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、磷脂酰胆碱（PC）。主要呼吸醌为泛醌 Q-8。基于上述多相分类，菌株 GXH0434 代表微杆菌属的一个新种，现提议将其命名为滨海微杆菌 Litoralis sp. nov.。模式菌株为 GXH0434（= MCCC 1K07158 = KCTC 92169）。
