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An optimized Alu-PCR primer pair for human-specific amplification of YACs and somatic cell hybrids.

作者信息

Tagle D A, Collins F S

机构信息

Department of Human Genetics, University of Michigan Medical Center, Ann Arbor 48109.

出版信息

Hum Mol Genet. 1992 May;1(2):121-2. doi: 10.1093/hmg/1.2.121.

DOI:10.1093/hmg/1.2.121
PMID:1301148
Abstract
摘要

相似文献

1
An optimized Alu-PCR primer pair for human-specific amplification of YACs and somatic cell hybrids.
Hum Mol Genet. 1992 May;1(2):121-2. doi: 10.1093/hmg/1.2.121.
2
Identification of region-specific yeast artificial chromosomes using pools of Alu element-mediated polymerase chain reaction probes labeled via linear amplification.
Genomics. 1992 Dec;14(4):931-8. doi: 10.1016/s0888-7543(05)80114-4.
3
Large-scale cloning of human chromosome 2-specific yeast artificial chromosomes (YACs) using an interspersed repetitive sequences (IRS)-PCR approach.使用散布重复序列(IRS)-PCR方法对人类2号染色体特异性酵母人工染色体(YAC)进行大规模克隆。
Genomics. 1995 Mar 20;26(2):178-91. doi: 10.1016/0888-7543(95)80199-v.
4
Cloning and characterization of EagI YACs from human chromosome 21.来自人类21号染色体的EagI酵母人工染色体的克隆与特性分析
Genomics. 1993 Jan;15(1):228-30. doi: 10.1006/geno.1993.1043.
5
Human chromosome 5 sequence primer amplifies Alu polymorphisms on chromosomes 2 and 17.人类5号染色体序列引物可扩增2号和17号染色体上的Alu多态性。
Genome. 1993 Apr;36(2):302-9. doi: 10.1139/g93-042.
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Alu-based vectorettes and splinkerettes. More efficient and comprehensive polymerase chain reaction amplification of human DNA from complex sources.
Genet Anal Tech Appl. 1994;11(4):95-101. doi: 10.1016/1050-3862(94)90046-9.
7
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification.Alu-PCR扩增后YAC克隆的荧光原位杂交。
Genomics. 1992 Jul;13(3):826-8. doi: 10.1016/0888-7543(92)90160-t.
8
A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods.一种通过STS和Alu-PCR方法进行文库筛选的二维酵母人工染色体(YAC)池化策略。
Nucleic Acids Res. 1992 May 25;20(10):2559-63. doi: 10.1093/nar/20.10.2559.
9
The human chromosome content in human x rodent somatic cell hybrids analyzed by a screening technique using Alu PCR.通过使用Alu PCR的筛选技术分析的人-啮齿动物体细胞杂种中的人类染色体含量。
Genomics. 1991 May;10(1):186-92. doi: 10.1016/0888-7543(91)90499-5.
10
Isolation of a human chromosome 14-only somatic cell hybrid: analysis using Alu and LINE-based PCR.
Genomics. 1991 Sep;11(1):215-8. doi: 10.1016/0888-7543(91)90122-u.

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Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes.鉴定出一个能逆转部分细胞永生表型的基因,该基因是一个新的类转录因子基因家族的成员。
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Genetic mapping refines DFNB3 to 17p11.2, suggests multiple alleles of DFNB3, and supports homology to the mouse model shaker-2.
基因定位将DFNB3精确定位于17p11.2,提示DFNB3存在多个等位基因,并支持其与小鼠模型shaker-2具有同源性。
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Angelman syndrome associated with an inversion of chromosome 15q11.2q24.3.与15号染色体q11.2q24.3倒位相关的天使综合征。
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Down syndrome: characterisation of a case with partial trisomy of chromosome 21 owing to a paternal balanced translocation (15;21) (q26;q22.1) by FISH.唐氏综合征:通过荧光原位杂交技术(FISH)对因父亲平衡易位(15;21)(q26;q22.1)导致21号染色体部分三体的一例病例的特征分析。
J Med Genet. 1997 Jan;34(1):50-4. doi: 10.1136/jmg.34.1.50.
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Cloning (CAG/GTC)n STSs by an Alu-(CAG/GTC)n PCR method: an approach to human chromosome 12 and spinocerebellar ataxia 2 (SCA2).通过Alu-(CAG/GTC)n聚合酶链式反应方法克隆(CAG/GTC)n序列标签位点:人类12号染色体及脊髓小脑共济失调2型(SCA2)的研究方法
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Generation of sequence-tagged sites from Xp22.3 by isolating common Alu-PCR products of radiation hybrids retaining overlapping human X chromosome fragments.通过分离保留重叠人类X染色体片段的辐射杂种的常见Alu-PCR产物,从Xp22.3生成序列标签位点。
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Island rescue PCR: a rapid and efficient method for isolating transcribed sequences from yeast artificial chromosomes and cosmids.岛式拯救PCR:一种从酵母人工染色体和黏粒中分离转录序列的快速高效方法。
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