牛3型腺病毒的分子克隆及限制性内切酶分析

Molecular cloning and restriction enzyme analysis of bovine adenovirus type 3.

作者信息

Elgadi M, Haj-Ahmad Y

机构信息

Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada.

出版信息

Intervirology. 1992;34(3):124-32. doi: 10.1159/000150272.

DOI:10.1159/000150272

PMID:1302248

Abstract

Bovine adenovirus type 3 (BAV3) is a DNA virus that causes respiratory and gastrointestinal disorders in cattle. The viral genome consists of a linear double-stranded DNA molecule (35,000 base pairs) with inverted terminal repeats at each of its 5' molecular ends. We have subcloned 10 HindIII fragments spanning 4.9-96.0%, 5 EcoRI fragments spanning 3.4-89.5% and 2 XbaI fragments spanning 35.7-82.9% of the BAV3 (strain WBR-1) genome into the bacterial cloning vector pUC19. The subcloning of the viral genome facilitated the construction of linear restriction enzyme maps for BamHI, ClaI, EcoRI, HindIII, KpnI, NotI, NspV, PstI, PvuI, SalI, XbaI and XhoI. In this study we report on the molecular cloning and restriction endonuclease mapping of the BAV3 genome.

摘要

牛3型腺病毒（BAV3）是一种DNA病毒，可引起牛的呼吸道和胃肠道疾病。病毒基因组由一个线性双链DNA分子（35,000个碱基对）组成，在其5'分子末端各有一个反向末端重复序列。我们已将跨越BAV3（WBR-1株）基因组4.9-96.0%的10个HindIII片段、跨越3.4-89.5%的5个EcoRI片段和跨越35.7-82.9%的2个XbaI片段亚克隆到细菌克隆载体pUC19中。病毒基因组的亚克隆有助于构建BamHI、ClaI、EcoRI、HindIII、KpnI、NotI、NspV、PstI、PvuI、SalI、XbaI和XhoI的线性限制酶图谱。在本研究中，我们报告了BAV3基因组的分子克隆和限制内切酶图谱。