Kurokawa T, Igarashi K, Sugino Y
J Virol. 1978 Oct;28(1):212-8. doi: 10.1128/JVI.28.1.212-218.1978.
Cleavage of bovine adenovirus type 3 (BAV3) DNA by restriction endonucleases EcoRI, BamHI, and HindIII yielded 7 (A to G), 5 (A to E), and 12 (A to L) fragments, respectively. The order of these fragments has been determined to be GDACBFE for EcoRI fragments, AEBDC for BamHI fragments, and JEBKACDHFGIL for HindIII fragments, and cleavage sites of these enzymes have been mapped on the genome of BAV3. BAV3 preparation contains incomplete virus whose genome has a deletion of about 13% of complete virus genome. Restriction endonuclease digestion of the incomplete virus DNA revealed that EcoRI E and F, BamHI C and HindIII G, I, and L fragments were deleted. Therefore, the deleted region of incomplete virus DNA is located near the right-hand end of the BAV3 DNA molecule, a result consistent with our previous electron-microscopic observations on heteroduplex molecules formed between complete and incomplete BAV3 DNA.
用限制性内切酶EcoRI、BamHI和HindIII切割牛3型腺病毒（BAV3）DNA，分别产生了7个（A至G）、5个（A至E）和12个（A至L）片段。已确定这些片段的顺序对于EcoRI片段为GDACBFE，对于BamHI片段为AEBDC，对于HindIII片段为JEBKACDHFGIL，并且这些酶的切割位点已定位在BAV3的基因组上。BAV3制剂包含基因组缺失约13%完整病毒基因组的不完整病毒。对不完整病毒DNA进行限制性内切酶消化显示，EcoRI的E和F片段、BamHI的C片段以及HindIII的G、I和L片段缺失。因此，不完整病毒DNA的缺失区域位于BAV3 DNA分子的右端附近，这一结果与我们之前对完整和不完整BAV3 DNA之间形成的异源双链分子的电子显微镜观察结果一致。
