Sánchez-Muniz F J, Cuesta C, San-Félix B
Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Rev Esp Fisiol. 1992 Dec;48(4):271-6.
Two different methods for low-density-lipoprotein-cholesterol (LDL-C) determination were comparatively used. The heparin-sodium citrate (pH 5.12) precipitation method gave similar LDL-C results to the ones given by the Friedewald et al. formula (3.2 vs 3.3 mmol/l) in 187 men. Values obtained using both methods show a very high and significant correlation (r > 0.9; p < 0.001). However, LDL-C values obtained with the precipitation method were 15% higher in hypertriglyceridemics (triglycerides (Tg) > or = 2.3 mmol/l). A paired-comparison between data obtained by both methods indicates a clear serum Tg-values influence, because LDL-C values obtained by the precipitation method were significantly more frequently higher (p < 0.05 or p < 0.01) than LDL-C values obtained using the Friedewald's formula in hypertriglyceridemic men (Tg > or = 1.7 mmol/l or Tg > or = 2.3 mmol/l respectively). When a 3.9 mmol/l LDL-C level break was chosen, Friedewald's formula gave 13% false hypercholesterolemics. The influence of Tg was again significant in men with both, hypercholesterolemia and hypertriglyceridemia, while LDL-C values obtained by the precipitation method were significantly more frequently higher (p < 0.01).
比较使用了两种不同的低密度脂蛋白胆固醇（LDL-C）测定方法。在187名男性中，肝素柠檬酸钠（pH 5.12）沉淀法得出的LDL-C结果与Friedewald等人的公式得出的结果相似（分别为3.2 mmol/l和3.3 mmol/l）。使用两种方法获得的值显示出非常高且显著的相关性（r > 0.9；p < 0.001）。然而，在高甘油三酯血症患者（甘油三酯（Tg）≥2.3 mmol/l）中，沉淀法获得的LDL-C值高15%。两种方法获得的数据进行配对比较表明，血清Tg值有明显影响，因为在高甘油三酯血症男性（分别为Tg≥1.7 mmol/l或Tg≥2.3 mmol/l）中，沉淀法获得的LDL-C值显著更频繁地高于使用Friedewald公式获得的LDL-C值（p < 0.05或p < 0.01）。当选择3.9 mmol/l的LDL-C水平界限时，Friedewald公式得出13%的假高胆固醇血症患者。在同时患有高胆固醇血症和高甘油三酯血症的男性中，Tg的影响再次显著，而沉淀法获得的LDL-C值显著更频繁地更高（p < 0.01）。
