Galbraith A M, Malone R E
University of Iowa, Iowa City.
Dev Genet. 1992;13(6):392-402. doi: 10.1002/dvg.1020130603.
The REC104 gene was initially defined by mutations that rescued the inviability of a rad52 spo 13 haploid strain in meiosis. We have observed that rec104 mutant strains undergo essentially no induction of meiotic gene conversion, and we have not been able to detect any meiotic crossing over in such strains. The REC104 gene has no apparent role in mitosis, since mutations have no observable effect on growth, mitotic recombination, or DNA repair. The DNA sequence of REC104 reveals that it is a previously unknown gene with a coding region of 549-bp, and genetic mapping has localized the gene to chromosome VIII near FUR1. Expression of the REC104 gene is induced in meiosis, and it appears that the gene is not transcribed in mitotic cells. Possible roles for the REC104 gene product in meiosis are discussed.
REC104基因最初是通过能挽救减数分裂中rad52 spo 13单倍体菌株致死性的突变来定义的。我们观察到rec104突变菌株基本上不会诱导减数分裂基因转换，并且我们在这类菌株中未能检测到任何减数分裂交换。REC104基因在有丝分裂中没有明显作用，因为突变对生长、有丝分裂重组或DNA修复没有可观察到的影响。REC104的DNA序列显示它是一个以前未知的基因，编码区为549个碱基对，遗传定位已将该基因定位于VIII号染色体上靠近FUR1的位置。REC104基因的表达在减数分裂中被诱导，并且该基因似乎不在有丝分裂细胞中转录。文中讨论了REC104基因产物在减数分裂中的可能作用。
