Frevert C W, Warner A E, Weller E, Brain J D
Physiology Program, Harvard School of Public Health, Boston, Massachusetts, USA.
Exp Lung Res. 1998 Nov-Dec;24(6):745-58. doi: 10.3109/01902149809099593.
This study was performed to explore whether alveolar macrophage (AM) phagocytosis would be impaired during endotoxemia. Therefore, we characterized in vivo AM phagocytic function in rats following either intravenous (i.v.) or intratracheal (i.t.) administration of lipopolysaccharide (LPS). The i.v. administration of LPS to rats at dosages of 0, 1, 2, and 5 mg/kg showed that increasing LPS doses were significantly associated with increased AM phagocytosis of 198Au colloid (P < .01), decreased recovery of AMs in bronchoalveolar lavage (BAL) (P = .017), no significant differences in neutrophil recovery by lavage (P = .15), or in the concentration of albumin in BAL (P = .14). Across the dosages of LPS administered i.t. (i.e., 0, 1, 5, and 10 mg/kg), there was no difference in AM phagocytosis (P = .29), a significant decrease in AM recovery (P = .002), a significant increase in neutrophil number (P = .01), and little effect on the concentration of albumin (P = .06). Thus, we found that the administration of endotoxin to rats did not impair in vivo AM phagocytic function. In fact, our findings suggest that the i.v. administration of LPS may increase AM phagocytosis of 198Au.
本研究旨在探讨内毒素血症期间肺泡巨噬细胞（AM）的吞噬功能是否会受损。因此，我们对静脉注射（i.v.）或气管内（i.t.）给予脂多糖（LPS）后的大鼠体内AM吞噬功能进行了表征。以0、1、2和5 mg/kg的剂量对大鼠静脉注射LPS的结果显示，LPS剂量增加与198Au胶体的AM吞噬作用增加显著相关（P <.01），支气管肺泡灌洗（BAL）中AM的回收率降低（P =.017），灌洗中性粒细胞回收率无显著差异（P =.15），BAL中白蛋白浓度也无显著差异（P =.14）。经气管内给予不同剂量的LPS（即0、1、5和10 mg/kg）后，AM吞噬作用无差异（P =.29），AM回收率显著降低（P =.002），中性粒细胞数量显著增加（P =.01），对白蛋白浓度影响不大（P =.06）。因此，我们发现给大鼠注射内毒素不会损害体内AM的吞噬功能。事实上，我们的研究结果表明，静脉注射LPS可能会增加AM对198Au的吞噬作用。
