ACKERMANN W W, JOHNSON R B
J Exp Med. 1953 Mar;97(3):315-22. doi: 10.1084/jem.97.3.315.
It was found that DNP (2,4-dinitrophenol) will inhibit completely the propagation of influenza virus in chorioallantoic membrane. This reagent did not permanently alter those metabolic processes required for the synthesis of virus and at the concentrations employed demonstrated no virucidal effects. In minced preparations of chorioallantoic membrane DNP was shown to have a pronounced stimulatory effect upon ATPase (adenosinetriphosphatase). When DNP was used with intact tissues, an excellent correlation was found between the inhibition of viral propagation and the stimulation of respiration and release of phosphate. Concentrations of DNP which permitted a twofold increase in the endogenous respiration of intact membranes allowed little or no viral synthesis. It is concluded that the energy required for viral synthesis derives from the oxidative phosphorylative activity of the host tissue.
已发现2,4-二硝基苯酚（DNP）能完全抑制流感病毒在绒毛尿囊膜中的增殖。该试剂不会永久性改变病毒合成所需的那些代谢过程，并且在所使用的浓度下未显示出杀病毒作用。在绒毛尿囊膜的切碎制剂中，DNP对三磷酸腺苷酶（ATPase）具有明显的刺激作用。当DNP与完整组织一起使用时，发现病毒增殖的抑制与呼吸刺激和磷酸盐释放之间存在良好的相关性。使完整膜的内源性呼吸增加两倍的DNP浓度几乎不允许病毒合成或完全不允许病毒合成。结论是病毒合成所需的能量来自宿主组织的氧化磷酸化活性。
