Tsurumi H, Shimazaki M, Takemura M, Shimizu N, Moriwaki H, Muto Y, Shimokawa K, Noma A
1st Department of Internal Medicine, Gifu University School of Medicine.
Rinsho Byori. 1992 Oct;40(10):1080-4.
We investigated active oxygen (H2O2) production by neutrophils in patients with inflammatory diseases such as connective tissue disease and respiratory tract infection. The analysis was done by flow cytometry using as small volume of whole-blood as 100 microliters. H2O2 production as well as C-reactive protein (CRP) were increased in connective tissue diseases, and was decreased after steroid therapy. In a case of neuro-Behçet's disease, changes in H2O2 production was observed in agreement with those in clinical symptoms such as gait disturbance. H2O2 production as well as CRP and neutrophil count were increased in acute respiratory tract infection, but in chronic patients H2O2 production alone was rather reduced. In stages of enhanced H2O2 production, tissue damage or inflammation may exist, the degree of which seems to be well reflected by H2O2 production. During stages of impaired H2O2 production, care should be taken to prevent infections. Using flow cytometry, H2O2 production can be easily determined as one of functions of neutrophils.
我们研究了结缔组织病和呼吸道感染等炎症性疾病患者中性粒细胞产生活性氧（H2O2）的情况。分析采用流式细胞术，使用的全血样本量低至100微升。结缔组织病患者的H2O2生成量以及C反应蛋白（CRP）均升高，且在接受类固醇治疗后降低。在1例神经白塞病患者中，观察到H2O2生成量的变化与步态障碍等临床症状的变化一致。急性呼吸道感染患者的H2O2生成量以及CRP和中性粒细胞计数均升高，但慢性患者仅H2O2生成量有所降低。在H2O2生成增强阶段，可能存在组织损伤或炎症，其程度似乎可通过H2O2生成量得到很好的反映。在H2O2生成受损阶段，应注意预防感染。使用流式细胞术，可以轻松测定H2O2生成量，将其作为中性粒细胞的功能之一。
