Yuan L, Inoue S, Saito Y, Nakajima O
Department of Environmental Medicine, Graduate School of Environmental Science, Hokkaido University Hospital, Hokkaido University, Sapporo, Japan.
Exp Cell Res. 1993 Dec;209(2):375-81. doi: 10.1006/excr.1993.1323.
To evaluate the effects of inflammatory cytokines on oxidative production in normal neutrophils, seven kinds of cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), interleukin-2 (IL-2), IL-6, IL-1 alpha, IL-1 beta, and interferon-beta (IFN-beta) were tested. The intracellular hydrogen peroxide (H2O2) in individual cells was determined by flow cytometry. According to the levels of intracellular H2O2 enhanced by cytokines, these seven cytokines were classified into three types: (1) prominently effective--GM-CSF; (2) moderately effective--G-CSF, IL-6, and IL-2; (3) weakly or ineffective--IFN-beta, IL-1 alpha, and IL-1 beta. Changes in cell size and cell surface structure after stimulation of those seven cytokines were simultaneously measured by flow cytometry. The most prominently effective cytokine, GM-CSF, initially caused enlargement of cell size and irregularity of the cell surface and subsequently increased H2O2 production by neutrophils. In contrast, the weakly or ineffective cytokines, like IL-1 beta, had no effects on cell size or cell surface. Our study indicates that some kinds of cytokines enhance oxidative production and cause morphological changes in neutrophils.
为评估炎性细胞因子对正常中性粒细胞氧化产物生成的影响，我们检测了七种细胞因子，如粒细胞-巨噬细胞集落刺激因子（GM-CSF）、粒细胞集落刺激因子（G-CSF）、白细胞介素-2（IL-2）、IL-6、IL-1α、IL-1β和干扰素-β（IFN-β）。通过流式细胞术测定单个细胞内的过氧化氢（H2O2）。根据细胞因子增强细胞内H2O2的水平，将这七种细胞因子分为三类：（1）显著有效——GM-CSF；（2）中度有效——G-CSF、IL-6和IL-2；（3）弱效或无效——IFN-β、IL-1α和IL-1β。通过流式细胞术同时测量这七种细胞因子刺激后细胞大小和细胞表面结构的变化。最显著有效的细胞因子GM-CSF最初导致细胞大小增大和细胞表面不规则，随后增加中性粒细胞的H2O2生成。相比之下，弱效或无效的细胞因子，如IL-1β，对细胞大小或细胞表面没有影响。我们的研究表明，某些细胞因子可增强中性粒细胞的氧化产物生成并引起其形态变化。
