Kellett M, Potten C S, Rew D A
Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Epithelial Cell Biol. 1992 Oct;1(4):147-55.
Using tritiated thymidine (3HTdR) labelling in vivo in the mouse we have determined the labelling index (LI%) at each cell position along the sides of sections of crypts in the small and large bowel. We have compared LI versus cell position frequency plots obtained in this way with those obtained using bromodeoxyuridine (BrdUrd) in vivo in the small intestine. Both thymidine analogues give identical patterns and similar levels of labelling: for example, the overall LI is 29.1% after 3HTdR and 34.7% after BrdUrd in the mouse ileum. Similar data have been obtained following in vivo labelling in humans with BrdUrd prior to gastrointestinal surgery for cancer and in mouse colon following 3HTdR labelling. Comparisons between the mouse and human data show that the spatial distribution of label within the crypts occurs at the same relative positions in the two species. However, the intestinal crypts are between 2-fold and 4-fold larger, particularly in their length, in the human: for example, 250 and 450 cells per crypt for ileum and 590 and 2000 cells per crypt for the colon in mouse and human respectively. The absolute value of the maximum LI in the mouse small intestine (56.5%) is higher than it is in the human (26.3%). However, the patterns of proliferation are similar in the two species under steady-state conditions.
我们通过在小鼠体内使用氚标记胸腺嘧啶核苷（³HTdR）进行标记，确定了小肠和大肠隐窝切片边缘每个细胞位置的标记指数（LI%）。我们将以这种方式获得的LI与细胞位置频率图，与在小肠中使用5-溴脱氧尿嘧啶核苷（BrdUrd）在体内获得的图进行了比较。两种胸腺嘧啶类似物给出了相同的模式和相似的标记水平：例如，在小鼠回肠中，³HTdR标记后的总体LI为29.1%，BrdUrd标记后为34.7%。在人类癌症患者进行胃肠手术前用BrdUrd进行体内标记后，以及在³HTdR标记后的小鼠结肠中，也获得了类似的数据。小鼠和人类数据的比较表明，隐窝内标记的空间分布在两个物种的相同相对位置出现。然而，人类的肠隐窝大2至4倍，特别是在长度方面：例如，小鼠和人类回肠每个隐窝分别为250和450个细胞，结肠每个隐窝分别为590和2000个细胞。小鼠小肠中最大LI的绝对值（56.5%）高于人类（26.3%）。然而，在稳态条件下，两个物种的增殖模式相似。
