Potten C S, Kellett M, Roberts S A, Rew D A, Wilson G D
CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester.
Gut. 1992 Jan;33(1):71-8. doi: 10.1136/gut.33.1.71.
In vivo bromodeoxyuridine (BrdUrd) labelling of the human large bowel was performed and a detailed histochemical localisation of label in sections of crypts was undertaken using a monoclonal antibody to BrdUrd containing DNA. Flow cytometric studies on extracted nuclei were also performed (data presented elsewhere). The average crypt in the human large bowel (excluding the rectum) was 82 cells in height and 41 cells in circumference, with a total of about 2000 cells (assuming a topographical correction factor of 0.6). Ten per cent of the cells were replicating their DNA--that is, were in the S phase of the cell cycle--and 0.4% were in mitosis. The median position for the labelling index versus cell position frequency plot is at the 20th cell position--at a quarter of the crypt height. The lower and upper limits of the cell proliferation are given by the 5th and 95th percentiles at cell positions 4 and 43 respectively. The peak labelling index is about 30% and it occurs at cell position 15. The labelling index at the crypt base, the probable stem cell zone, is about 14%, suggesting that these cells have a longer cell cycle. Taking a value of 8.6 hours for the duration of the S phase (deduced from the flow cytometric data) and assuming a growth fraction of 1.0 for the mid-crypt, these data provide an estimate of about 30 hours for the cell cycle time. The rectal crypts are about the same size but contain about 30% fewer S phase cells. The data also yielded a per cent BrdUrd labelled mitosis curve.
对人大肠进行了体内溴脱氧尿苷（BrdUrd）标记，并使用针对含有BrdUrd的DNA的单克隆抗体对隐窝切片中的标记进行了详细的组织化学定位。还对提取的细胞核进行了流式细胞术研究（数据在其他地方呈现）。人大肠（不包括直肠）的平均隐窝高度为82个细胞，周长为41个细胞，总共约有2000个细胞（假设地形校正因子为0.6）。10%的细胞正在复制其DNA，即处于细胞周期的S期，0.4%的细胞处于有丝分裂期。标记指数与细胞位置频率图的中位数位置在第20个细胞位置，即隐窝高度的四分之一处。细胞增殖的下限和上限分别由细胞位置4和43处的第5和第95百分位数给出。峰值标记指数约为30%，出现在细胞位置15处。隐窝底部（可能的干细胞区）的标记指数约为14%，这表明这些细胞的细胞周期较长。以S期持续时间8.6小时（从流式细胞术数据推导得出）并假设隐窝中部的生长分数为1.0，这些数据提供了细胞周期时间约为30小时的估计值。直肠隐窝大小大致相同，但S期细胞数量少约30%。这些数据还得出了BrdUrd标记有丝分裂曲线的百分比。
