Rodriguez J M, Salas M L, Viñuela E
Centro de Biologia Molecular, (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Virology. 1992 Jan;186(1):40-52. doi: 10.1016/0042-6822(92)90059-x.
The nucleotide sequence of the 6004-bp EcoRI I fragment of African swine fever virus DNA has been determined. Translation of the sequence revealed eight closely spaced open reading frames (ORFs), three of them reading rightward and five leftward. Northern blot hybridization analysis indicated that ORFs I73R and I78R were transcribed early in infection, whereas ORFs I177L, I196L, and I329L were expressed at late times. Transcripts for ORFs I215L, I226R, and I243L were detected at low levels in early RNA and at higher levels in late RNA. The intergenic regions between genes I73R/I329L and I78R/I215L were characterized by the presence of direct repeats in tandem. Direct repetitions were also found within ORF I196L. The protein encoded by ORF I329L contained a putative cleavable signal peptide and an internal transmembrane domain, and that encoded by ORF I177L had an amino-terminal hydrophobic region with the characteristics of a "start-stop" sequence. ORF I243L encoded a protein similar to the eukaryotic elongation factor SII. The protein encoded by ORF I215L was homologous to the family of ubiquitin-conjugating proteins.
已确定非洲猪瘟病毒DNA的6004碱基对EcoRI I片段的核苷酸序列。对该序列进行翻译后发现有8个紧密排列的开放阅读框（ORF），其中3个向右读码，5个向左读码。Northern印迹杂交分析表明，ORF I73R和I78R在感染早期转录，而ORF I177L、I196L和I329L在感染后期表达。在早期RNA中低水平检测到ORF I215L、I226R和I243L的转录本，在晚期RNA中高水平检测到。基因I73R/I329L和I78R/I215L之间的基因间隔区的特征是存在串联直接重复序列。在ORF I196L内也发现了直接重复序列。ORF I329L编码的蛋白质含有一个推定的可切割信号肽和一个内部跨膜结构域，ORF I177L编码的蛋白质具有一个带有“起始-终止”序列特征的氨基末端疏水区域。ORF I243L编码一种类似于真核延伸因子SII的蛋白质。ORF I215L编码的蛋白质与泛素结合蛋白家族同源。
