Flemington E K, Borras A M, Lytle J P, Speck S H
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
J Virol. 1992 Feb;66(2):922-9. doi: 10.1128/JVI.66.2.922-929.1992.
Initiation of the Epstein-Barr virus (EBV) lytic cycle is dependent on expression of the viral transactivator Zta, which is encoded by the BZLF1 gene. Described here is an initial mapping of the regions of Zta involved in activating transcription. The data indicate that the amino-terminal 153 amino acids of Zta are important for activity, and in particular the region from residues 28 to 78 appears to be critical for Zta function. However, other features of Zta may be important for activity since a Gal4-Zta chimeric protein, generated by fusing the amino-terminal 167 residues of Zta to the DNA binding domain of the yeast transactivator Gal4, transactivated a minimal promoter containing one upstream Gal4 binding site but was unable to exhibit synergistic transactivation when assayed with a reporter containing five upstream Gal4 binding sites.
爱泼斯坦-巴尔病毒（EBV）裂解周期的启动依赖于病毒反式激活因子Zta的表达，Zta由BZLF1基因编码。本文描述了Zta中参与激活转录区域的初步定位。数据表明，Zta的氨基末端153个氨基酸对活性很重要，特别是28至78位残基的区域似乎对Zta功能至关重要。然而，Zta的其他特征可能对活性也很重要，因为通过将Zta的氨基末端167个残基与酵母反式激活因子Gal4的DNA结合结构域融合而产生的Gal4-Zta嵌合蛋白，可激活含有一个上游Gal4结合位点的最小启动子，但在用含有五个上游Gal4结合位点的报告基因进行检测时，无法表现出协同反式激活作用。
