Expression of feline immunodeficiency virus gag gene in Escherichia coli.
作者信息
Furuya T, Hasegawa A, Saitoh M, Miyazawa T, Tohya Y, Hayami M, Takahashi E, Miki K, Mikami T
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
出版信息
Arch Virol. 1992;122(3-4):383-90. doi: 10.1007/BF01317200.
PMID:1309999
Abstract
The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.
摘要
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