Talbott R L, Sparger E E, Lovelace K M, Fitch W M, Pedersen N C, Luciw P A, Elder J H
Department of Molecular Biology, Research Foundation of Scripps Clinic, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5743-7. doi: 10.1073/pnas.86.15.5743.
An infectious molecular clone of the Petaluma strain of feline immunodeficiency virus (FIV) was isolated from a recombinant bacteriophage library containing genomic DNA prepared from FIV-infected Crandall feline kidney (CRFK) cells. The integrated provirus has a total length of 9472 base pairs. Three long open reading frames corresponding to GAG, POL, and ENV gene coding frames are evident. In addition, an open reading frame overlaps the 3' end of POL, in the region that encodes viral infectivity factor in the primate viruses. Several short open reading frames are present in the intergenic region between POL and ENV and within ENV, which may serve as exons for production of TAT and REV equivalents in FIV. Alignment of the predicted amino acid sequences of the FIV proteins with those of other lentiviruses indicates that FIV did not arise recently from any other characterized lentivirus.
从一个重组噬菌体文库中分离出猫免疫缺陷病毒（FIV）帕塔卢马毒株的感染性分子克隆，该文库包含从感染FIV的克兰德尔猫肾（CRFK）细胞制备的基因组DNA。整合的前病毒全长9472个碱基对。对应于GAG、POL和ENV基因编码框的三个长开放阅读框很明显。此外，一个开放阅读框与POL的3'端重叠，该区域在灵长类病毒中编码病毒感染因子。在POL和ENV之间的基因间隔区以及ENV内存在几个短开放阅读框，它们可能作为外显子用于在FIV中产生TAT和REV等效物。FIV蛋白预测氨基酸序列与其他慢病毒的氨基酸序列比对表明，FIV并非最近从任何其他已鉴定的慢病毒中产生。
