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Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein.

作者信息

Huang L, Tsui P, Freundlich M

机构信息

Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215.

出版信息

J Bacteriol. 1992 Feb;174(3):664-70. doi: 10.1128/jb.174.3.664-670.1992.

DOI:10.1128/jb.174.3.664-670.1992
PMID:1310090
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206141/
Abstract

The ompB operon encodes OmpR and EnvZ, two proteins that are necessary for the expression and osmoregulation of the OmpF and OmpC porins in Escherichia coli. We have used in vitro and in vivo experiments to show that cyclic AMP and the cyclic AMP receptor protein (CRP) directly regulate ompB. ompB expression in an ompB-lacZ chromosomal fusion strain was increased two- to fivefold when cells were grown in medium containing poor carbon sources or with added cyclic AMP. In vivo primer extension analysis indicated that this control is complex and involves both positive and negative effects by cyclic AMP-CRP on multiple ompB promoters. In vitro footprinting showed that cyclic AMP-CRP binds to a 34-bp site centered at -53 and at -75 in relation to the start sites of the major transcripts that are inhibited and activated, respectively, by this complex. Site-directed mutagenesis of the crp binding site provided evidence that this site is necessary for the in vivo regulation of ompB expression by cyclic AMP. Control of the ompB operon by cyclic AMP-CRP may account for the observed regulation of the formation of OmpF and OmpC by this complex (N. W. Scott and C. R. Harwood, FEMS Microbiol. Lett. 9:95-98, 1980).

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/69e19682de9b/jbacter00069-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/dd81ef67c613/jbacter00069-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/f00cb1ef9e77/jbacter00069-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/42a688474214/jbacter00069-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/69e19682de9b/jbacter00069-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/dd81ef67c613/jbacter00069-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/f00cb1ef9e77/jbacter00069-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/42a688474214/jbacter00069-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ca3/206141/69e19682de9b/jbacter00069-0024-a.jpg

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1
Positive and negative control of ompB transcription in Escherichia coli by cyclic AMP and the cyclic AMP receptor protein.
J Bacteriol. 1992 Feb;174(3):664-70. doi: 10.1128/jb.174.3.664-670.1992.
2
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本文引用的文献

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Temperate Bacteriophage Which Causes the Production of a New Major Outer Membrane Protein by Escherichia coli.导致大肠杆菌产生一种新的主要外膜蛋白的温和噬菌体。
J Virol. 1975 May;15(5):1121-30. doi: 10.1128/JVI.15.5.1121-1130.1975.
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Catabolite repression.分解代谢物阻遏
Cold Spring Harb Symp Quant Biol. 1961;26:249-56. doi: 10.1101/sqb.1961.026.01.031.
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The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12.大肠杆菌K12的ompB基因座与主要外膜孔蛋白的调控
Sci Adv. 2023 Mar 10;9(10):eade8582. doi: 10.1126/sciadv.ade8582. Epub 2023 Mar 8.
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Comprehensive analysis of central carbon metabolism illuminates connections between nutrient availability, growth rate, and cell morphology in Escherichia coli.全面分析中心碳代谢揭示了营养物质可用性、生长速率和大肠杆菌细胞形态之间的联系。
PLoS Genet. 2018 Feb 12;14(2):e1007205. doi: 10.1371/journal.pgen.1007205. eCollection 2018 Feb.
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Unexpected properties of sRNA promoters allow feedback control via regulation of a two-component system.小RNA启动子的意外特性允许通过双组分系统的调控实现反馈控制。
Nucleic Acids Res. 2016 Nov 16;44(20):9650-9666. doi: 10.1093/nar/gkw642. Epub 2016 Jul 20.
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Metabolic Regulation of a Bacterial Cell System with Emphasis on Escherichia coli Metabolism.细菌细胞系统的代谢调控，重点是大肠杆菌的代谢
ISRN Biochem. 2013 Feb 18;2013:645983. doi: 10.1155/2013/645983. eCollection 2013.
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OmpR, a response regulator of the two-component signal transduction pathway, influences inv gene expression in Yersinia enterocolitica O9.OmpR 是双组分信号转导途径的反应调节剂，影响肠侵袭性大肠杆菌 O9 的 inv 基因表达。
Front Cell Infect Microbiol. 2012 Dec 18;2:153. doi: 10.3389/fcimb.2012.00153. eCollection 2012.
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Different evolutionary modifications as a guide to rewire two-component systems.作为重新连接双组分系统指南的不同进化修饰
Bioinform Biol Insights. 2012;6:97-128. doi: 10.4137/BBI.S9356. Epub 2012 May 3.
9
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis.cAMP 受体蛋白 (CRP) 对鼠疫耶尔森氏菌孔蛋白基因及其自身基因的调控作用。
BMC Microbiol. 2011 Feb 23;11:40. doi: 10.1186/1471-2180-11-40.
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Phenotypic and transcriptional analysis of the osmotic regulator OmpR in Yersinia pestis.鼠疫耶尔森氏菌渗透调节剂 OmpR 的表型和转录分析。
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Genetic analysis of the ompB locus in Escherichia coli K-12.大肠杆菌K-12中ompB基因座的遗传分析。
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Cyclic AMP receptor protein: role in transcription activation.环磷酸腺苷受体蛋白：在转录激活中的作用。
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Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
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8
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10
Selection of lac gene fusions in vivo: ompR-lacZ fusions that define a functional domain of the ompR gene product.体内lac基因融合体的选择：界定ompR基因产物功能域的ompR-lacZ融合体。
J Bacteriol. 1984 Aug;159(2):750-6. doi: 10.1128/jb.159.2.750-756.1984.