使用与特定信使核糖核酸（mRNA）互补的核糖核酸（RNA）来调节单个细菌基因的表达。

The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes.

作者信息

Coleman J, Green P J, Inouye M

出版信息

Cell. 1984 Jun;37(2):429-36. doi: 10.1016/0092-8674(84)90373-8.

PMID:6202422

Abstract

A naturally occurring small RNA molecule ( micF RNA), complementary to the region encompassing the Shine-Dalgarno sequence and initiation codon of the ompF mRNA, is known to block the expression of that mRNA in E. coli. We have constructed a plasmid that produces a complementary RNA to the E. coli lpp mRNA (mic[Ipp] RNA). Induction of the mic(Ipp) gene efficiently blocked lipoprotein production and reduced the amount of lpp mRNA. Two mic(ompC) genes were similarly engineered and their expression was found to inhibit drastically production of OmpC. Analysis of several types of mic(ompA) genes suggests that micRNAs complementary to regions of the mRNA likely to come in contact with ribosomes were most effective. The novel capabilities of this artificial mic system provide great potential for application in both procaryotic and eucaryotic cells.

摘要

一种天然存在的小RNA分子（micF RNA），与包含ompF mRNA的Shine-Dalgarno序列和起始密码子的区域互补，已知其可在大肠杆菌中阻断该mRNA的表达。我们构建了一个质粒，该质粒可产生与大肠杆菌lpp mRNA互补的RNA（mic[Ipp] RNA）。mic（Ipp）基因的诱导有效阻断了脂蛋白的产生，并减少了lpp mRNA的量。两个mic（ompC）基因也以类似方式构建，发现它们的表达能显著抑制OmpC的产生。对几种类型的mic（ompA）基因的分析表明，与mRNA中可能与核糖体接触的区域互补的micRNAs最为有效。这种人工mic系统的新功能在原核细胞和真核细胞中都具有巨大的应用潜力。