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在双带果蝇基因组中鉴定出一个完整的P因子。

Identification of a complete P-element in the genome of Drosophila bifasciata.

作者信息

Hagemann S, Miller W J, Pinsker W

机构信息

Institut für Allgemeine Biologie, Abt. Genetik, Wien, Austria.

出版信息

Nucleic Acids Res. 1992 Feb 11;20(3):409-13. doi: 10.1093/nar/20.3.409.

DOI:10.1093/nar/20.3.409
PMID:1311074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310400/
Abstract

A full-size P-element (IbifM3) was isolated from a genomic library of Drosophila bifasciata. The sequence has a length of 2935 bp and is flanked by 8 bp duplications of the target site. The termini are formed by 31 bp inverted repeats. The four exons have intact reading frames and possess the coding capacity for a protein of 753 amino acids and a molecular weight of 86.4 kd. The sections of the D. melanogaster transposase presumed to be functionally important (three leucine zippers and a helix turn helix motif) are conserved in the D. bifasciata P-element. Copy number and genomic distribution resemble the situation in true P-strains of D. melanogaster. Both findings support the idea that IbifM3 represents an active transposon. The sequence comparison between the P-elements of D. bifasciata, D. melanogaster and Scaptomyza pallida reveals relationships not in accordance with the phylogeny of the species. This result suggests a further case of horizontal transmission involving mobile elements in the genus Drosophila.

摘要

从双带果蝇的基因组文库中分离出一个全长P元件（IbifM3）。该序列长度为2935 bp，两侧是靶位点的8 bp重复序列。末端由31 bp的反向重复序列形成。四个外显子具有完整的阅读框，具有编码753个氨基酸、分子量为86.4 kd的蛋白质的能力。黑腹果蝇转座酶中推测具有功能重要性的部分（三个亮氨酸拉链和一个螺旋-转角-螺旋基序）在双带果蝇P元件中是保守的。拷贝数和基因组分布类似于黑腹果蝇真正P品系中的情况。这两个发现都支持IbifM3代表一个活跃转座子的观点。双带果蝇、黑腹果蝇和苍白黑腹果蝇P元件之间的序列比较揭示了与物种系统发育不一致的关系。这一结果表明在果蝇属中存在涉及移动元件的水平转移的另一个例子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c13c/310400/abf0c9aee2ea/nar00077-0034-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c13c/310400/19a31ab21867/nar00077-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c13c/310400/abf0c9aee2ea/nar00077-0034-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c13c/310400/19a31ab21867/nar00077-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c13c/310400/abf0c9aee2ea/nar00077-0034-b.jpg

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本文引用的文献

1
Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination.黑腹果蝇杂种不育现象：一种包括突变、不育和雄性重组等异常特征的综合征。
Genetics. 1977 Aug;86(4):813-33. doi: 10.1093/genetics/86.4.813.
2
The P family of transposable elements in Drosophila.果蝇中的P家族转座因子
Annu Rev Genet. 1983;17:315-44. doi: 10.1146/annurev.ge.17.120183.001531.
3
Lambda replacement vectors carrying polylinker sequences.携带多克隆位点序列的λ置换载体。
生命之根：后生动物如何？
Front Cell Infect Microbiol. 2012 Apr 11;2:50. doi: 10.3389/fcimb.2012.00050. eCollection 2012.
4
New Drosophila P-like elements and reclassification of Drosophila P-elements subfamilies.新型果蝇 P 样元件和果蝇 P 元件亚家族的重新分类。
Mol Genet Genomics. 2012 Jul;287(7):531-40. doi: 10.1007/s00438-012-0691-y. Epub 2012 May 20.
5
Is the evolutionary history of the O-type P element in the saltans and willistoni groups of Drosophila similar to that of the canonical P element?果蝇盐沼种组和威氏种组中O型P因子的进化史与典型P因子的进化史相似吗？
J Mol Evol. 2007 Dec;65(6):715-24. doi: 10.1007/s00239-007-9051-7. Epub 2007 Nov 22.
6
Transposon display supports transpositional activity of P elements in species of the saltans group of Drosophila.转座子展示技术支持果蝇盐渍组物种中P因子的转座活性。
J Genet. 2007 Apr;86(1):37-43. doi: 10.1007/s12041-007-0005-z.
7
Canonical P elements are transcriptionally active in the saltans group of Drosophila.典型P因子在果蝇的盐渍果蝇组中具有转录活性。
J Mol Evol. 2004 Jul;59(1):31-40. doi: 10.1007/s00239-004-2601-3.
8
A phylogenetic perspective on P transposable element evolution in Drosophila.果蝇中P转座因子进化的系统发育视角
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11428-33. doi: 10.1073/pnas.94.21.11428.
9
Repeated horizontal transfer of P transposons between Scaptomyza pallida and Drosophila bifasciata.P 转座子在苍白菌潜蝇和双带果蝇之间的反复水平转移。
Genetica. 1996 Jul;98(1):43-51. doi: 10.1007/BF00120217.
10
A role for the KP leucine zipper in regulating P element transposition in Drosophila melanogaster.KP亮氨酸拉链在调控黑腹果蝇P因子转座中的作用。
Genetics. 1995 Oct;141(2):587-94. doi: 10.1093/genetics/141.2.587.
J Mol Biol. 1983 Nov 15;170(4):827-42. doi: 10.1016/s0022-2836(83)80190-9.
4
Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome.果蝇基因组中P转座因子的结构及其插入和切除位点。
Cell. 1983 Aug;34(1):25-35. doi: 10.1016/0092-8674(83)90133-2.
5
Evolution of hybrid dysgenesis determinants in Drosophila melanogaster.黑腹果蝇中杂种不育决定因子的进化
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1655-9. doi: 10.1073/pnas.80.6.1655.
6
The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family.P-M杂种不育的分子基础：P因子（一种P品系特异性转座子家族）的作用。
Cell. 1982 Jul;29(3):995-1004. doi: 10.1016/0092-8674(82)90463-9.
7
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。
Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.
8
Analysis of P transposable element functions in Drosophila.果蝇中P转座因子功能的分析。
Cell. 1984 Aug;38(1):135-46. doi: 10.1016/0092-8674(84)90534-8.
9
Apparent absence of transposable elements related to the P elements of D. melanogaster in other species of Drosophila.在果蝇的其他物种中明显不存在与黑腹果蝇P因子相关的转座元件。
Nature. 1984;310(5975):330-2. doi: 10.1038/310330a0.
10
Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing.果蝇P因子转座的组织特异性在mRNA剪接水平受到调控。
Cell. 1986 Jan 17;44(1):7-19. doi: 10.1016/0092-8674(86)90480-0.