Paricio N, Pérez-Alonso M, Martínez-Sebastián M J, de Frutos R
Department of Genetics, University of Valencia, Spain.
Nucleic Acids Res. 1991 Dec 25;19(24):6713-8. doi: 10.1093/nar/19.24.6713.
Several P homologous sequences have been cloned and sequenced from Drosophila subobscura. These sequences are located at the 85DE region of the O chromosome and at least three of them are organized in tandem. We have identified four copies which exhibit strong similarity between them. All of the isolated elements are truncated at the 5' and 3' ends. They have lost the inverted terminal repeats and exon 3, but maintain exons 0, 1 and 2. They are transcribed producing a polyadenylated RNA. The structure of these transcripts suggests that they are able to encode a 66 kd repressor-like protein, but not a functional transposase. We ask about the biological role of a potential repressor protein in this species.
从暗果蝇中克隆并测序了几个与P元件同源的序列。这些序列位于O染色体的85DE区域，其中至少有三个是串联排列的。我们鉴定出了四个彼此之间具有高度相似性的拷贝。所有分离出的元件在5'和3'末端均被截断。它们失去了反向末端重复序列和外显子3，但保留了外显子0、1和2。它们被转录产生多聚腺苷酸化的RNA。这些转录本的结构表明它们能够编码一种66kd的阻遏蛋白样蛋白，但不能编码功能性转座酶。我们探讨了这种潜在的阻遏蛋白在该物种中的生物学作用。
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