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单纯疱疹病毒1型糖蛋白B在昆虫细胞中的表达。对其生化和免疫学特性的初步分析。

Expression of herpes simplex virus type 1 glycoprotein B in insect cells. Initial analysis of its biochemical and immunological properties.

作者信息

Ghiasi H, Kaiwar R, Nesburn A B, Wechsler S L

机构信息

Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, CA 90048.

出版信息

Virus Res. 1992 Jan;22(1):25-39. doi: 10.1016/0168-1702(92)90087-p.

Abstract

A recombinant baculovirus (vAc-gB1) was constructed which expresses the glycoprotein B (gB) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with these recombinant viruses, a protein that was close in size to authentic HSV-1 gB was detected by gB polyclonal antibody. The recombinant gB was found on the membrane of Sf9 cells and was susceptible to tunicamycin, glycosidase F (PNGase F) and partially susceptible to Endo-H. Antibodies raised in mice to this recombinant recognized viral gB and neutralized the infectivity of HSV-1 in vitro. Mice inoculated with the recombinant gB were protected from lethal challenge with HSV-1.

摘要

构建了一种表达单纯疱疹病毒1型(HSV-1)糖蛋白B(gB)基因的重组杆状病毒(vAc-gB1)。当用这些重组病毒感染Sf9细胞时,gB多克隆抗体检测到一种大小与天然HSV-1 gB相近的蛋白质。重组gB存在于Sf9细胞的膜上,对衣霉素、糖苷酶F(PNGase F)敏感,对内切糖苷酶H部分敏感。用该重组蛋白免疫小鼠产生的抗体可识别病毒gB并在体外中和HSV-1的感染性。接种重组gB的小鼠可免受HSV-1致死性攻击。

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