Ghiasi H, Kaiwar R, Nesburn A B, Wechsler S L
Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
Virus Res. 1992 Jan;22(1):25-39. doi: 10.1016/0168-1702(92)90087-p.
A recombinant baculovirus (vAc-gB1) was constructed which expresses the glycoprotein B (gB) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with these recombinant viruses, a protein that was close in size to authentic HSV-1 gB was detected by gB polyclonal antibody. The recombinant gB was found on the membrane of Sf9 cells and was susceptible to tunicamycin, glycosidase F (PNGase F) and partially susceptible to Endo-H. Antibodies raised in mice to this recombinant recognized viral gB and neutralized the infectivity of HSV-1 in vitro. Mice inoculated with the recombinant gB were protected from lethal challenge with HSV-1.
构建了一种表达单纯疱疹病毒1型（HSV-1）糖蛋白B（gB）基因的重组杆状病毒（vAc-gB1）。当用这些重组病毒感染Sf9细胞时，gB多克隆抗体检测到一种大小与天然HSV-1 gB相近的蛋白质。重组gB存在于Sf9细胞的膜上，对衣霉素、糖苷酶F（PNGase F）敏感，对内切糖苷酶H部分敏感。用该重组蛋白免疫小鼠产生的抗体可识别病毒gB并在体外中和HSV-1的感染性。接种重组gB的小鼠可免受HSV-1致死性攻击。
