Cantin E M, Eberle R, Baldick J L, Moss B, Willey D E, Notkins A L, Openshaw H
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5908-12. doi: 10.1073/pnas.84.16.5908.
The herpes simplex virus 1 (HSV-1) strain F gene encoding glycoprotein gB was isolated and modified at the 5' end by in vitro oligonucleotide-directed mutagenesis. The modified gB gene was inserted into the vaccinia virus genome and expressed under the control of a vaccinia virus promoter. The mature gB glycoprotein produced by the vaccinia virus recombinant was glycosylated, was expressed at the cell surface, and was indistinguishable from authentic HSV-1 gB in terms of electrophoretic mobility. Mice immunized intradermally with the recombinant vaccinia virus produced gB-specific neutralizing antibodies and were resistant to a lethal HSV-1 challenge.
编码糖蛋白gB的单纯疱疹病毒1型（HSV-1）F株基因通过体外寡核苷酸定向诱变在5'端进行了分离和修饰。修饰后的gB基因被插入痘苗病毒基因组，并在痘苗病毒启动子的控制下表达。痘苗病毒重组体产生的成熟gB糖蛋白进行了糖基化修饰，在细胞表面表达，并且在电泳迁移率方面与天然HSV-1 gB没有区别。用重组痘苗病毒进行皮内免疫的小鼠产生了gB特异性中和抗体，并对致死性HSV-1攻击具有抗性。
