大肠杆菌gyrB基因的DNA序列：一种新测序策略的应用
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
作者信息
Adachi T, Mizuuchi M, Robinson E A, Appella E, O'Dea M H, Gellert M, Mizuuchi K
出版信息
Nucleic Acids Res. 1987 Jan 26;15(2):771-84. doi: 10.1093/nar/15.2.771.
Abstract
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene.
摘要
我们采用了一种新的测序方法确定了大肠杆菌gyrB基因的序列，该方法利用从微型Mu质粒到DNA的转座作用为双脱氧核苷酸序列分析提供随机起始点。gyrB序列对应的蛋白质长804个氨基酸；先前分离出的具有部分酶活性的蛋白质片段已被鉴定为C端半分子。gyrB转录的一个合理终止子位于结构基因之外。
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