Bleiweiss I J, Heller D, Dottino P, Cass I, Deligdisch L
Department of Pathology, Mount Sinai Medical Center, New York, NY 10029.
J Reprod Med. 1992 Feb;37(2):151-6.
To test the utility of biotinylated DNA probes against various subtypes of human papillomavirus (HPV), we performed in situ DNA hybridization on routinely processed archival material from 30 patients with serial cervical biopsies including conization (group I) and a prospective group of 35 patients whose cervical biopsies showed various degrees of koilocytotic atypia and/or dysplasia (group II). Commercially available biotinylated probe cocktails against HPV types 6 and 11, 16 and 18, and 31, 35 and 51 were detected via the avidin-biotin horseradish peroxidase technique. Virus was found in 87% (26/30) of group I and 57% (20/35) of group II. Almost exclusively, viral types 16, 18, 31, 35 and 51 were detected in group I; 54% (19/35) of group II stained for types 16, 18 or 31, 35 and 51; 2.9% (1/35) stained for types 6 and 11. Nine percent of group II (3/35) showed coinfection with types 16, 18 and 31, 35 and 51. Three of six vulvar condylomata (50%) stained for types 6 and 11. In general, weaker staining was associated with greater dysplasia. In situ hybridization using biotinylated DNA probes is useful in identifying patients infected with dysplasia/carcinoma-associated HPV subtypes and can be performed easily on routine surgical specimens.
为了测试生物素化DNA探针针对人乳头瘤病毒（HPV）各种亚型的效用，我们对30例接受包括锥切术在内的系列宫颈活检的患者（第一组）的常规处理存档材料以及35例宫颈活检显示不同程度挖空细胞异型性和/或发育异常的前瞻性患者组（第二组）进行了原位DNA杂交。通过抗生物素蛋白-生物素辣根过氧化物酶技术检测针对HPV 6型和11型、16型和18型以及31型、35型和51型的市售生物素化探针混合物。在第一组中87%（26/30）发现病毒，在第二组中57%（20/35）发现病毒。在第一组中几乎仅检测到16型、18型、31型、35型和51型病毒；第二组中54%（19/35）的标本16型、18型或31型、35型和51型呈阳性染色；2.9%（1/35）的标本6型和11型呈阳性染色。第二组中有9%（3/35）显示16型、18型与31型、35型和51型合并感染。6例外阴湿疣中有3例（50%）6型和11型呈阳性染色。一般来说，染色较弱与发育异常程度较高相关。使用生物素化DNA探针的原位杂交有助于识别感染发育异常/癌相关HPV亚型的患者，并且可以在常规手术标本上轻松进行。
