Clarke L M, Daidone B J, Inghida R, Kirwin M, Sierra M F
Department of Pathology, SUNY Health Science Center, Brooklyn 11203.
Am J Clin Pathol. 1992 Mar;97(3):313-7. doi: 10.1093/ajcp/97.3.313.
The recovery of cytomegalovirus from bronchoalveolar lavage (BAL) specimens was compared after inoculation of MRC-5 tube and shell vial cell cultures with four different BAL preparations. Analysis of culture results obtained with 55 cytomegalovirus culture-positive samples showed significant differences in the ability to isolate virus from the supernatant and cellular components of these specimens. There was a 52% reduction in cytomegalovirus recovery and a significant delay in the development of cytopathic effect in cultures inoculated with the cellular component of BAL specimens when compared to cultures inoculated with crude BAL cells and fluid. The mean time for detection of cytopathic effect was 11.8 days in tubes inoculated with crude BAL and 18.2 days for tubes inoculated with BAL cells. A similar effect was observed using a rapid shell vial culture technique. A 39% reduction in the number of isolates and a 57% reduction in the number of positive cells were observed in vials inoculated with cells when compared to cultures inoculated with crude BAL. By contrast, using cell-free BAL supernatant as inoculum did not reduce the number of positive cultures or delay development of cytopathic effect. The results suggest that in most BAL specimens, cytomegalovirus is associated with the cell-free, rather than the cellular, component. Although BAL cell concentrates frequently are used for cultivation of viruses from BAL, our results showed that the use of these preparations results in a significant number of false-negative cytomegalovirus cultures.
用四种不同的支气管肺泡灌洗（BAL）制剂接种MRC - 5试管和空斑试验瓶细胞培养物后，比较了从BAL标本中分离出巨细胞病毒的情况。对55份巨细胞病毒培养阳性样本的培养结果分析表明，从这些标本的上清液和细胞成分中分离病毒的能力存在显著差异。与接种粗制BAL细胞和液体的培养物相比，接种BAL标本细胞成分的培养物中巨细胞病毒回收率降低了52%，细胞病变效应出现的时间显著延迟。接种粗制BAL的试管中检测到细胞病变效应的平均时间为11.8天，接种BAL细胞的试管为18.2天。使用快速空斑试验瓶培养技术也观察到了类似的效果。与接种粗制BAL的培养物相比，接种细胞的试验瓶中分离株数量减少了39%，阳性细胞数量减少了57%。相比之下，使用无细胞BAL上清液作为接种物并没有减少阳性培养物的数量，也没有延迟细胞病变效应的出现。结果表明，在大多数BAL标本中，巨细胞病毒与无细胞成分而非细胞成分相关。尽管BAL细胞浓缩物经常用于从BAL中培养病毒，但我们的结果表明，使用这些制剂会导致大量巨细胞病毒培养出现假阴性。
