Storch G A, Ettinger N A, Ockner D, Wick M R, Gaudreault-Keener M, Rossiter J, Trulock E P, Cooper J D
Dept. of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, MO 63110.
J Infect Dis. 1993 Dec;168(6):1502-6. doi: 10.1093/infdis/168.6.1502.
Results of quantitative shell vial cultures of cell and supernatant fractions of bronchoalveolar lavage (BAL) fluid from lung transplant recipients were compared with results of transbronchial lung biopsies. Cytomegalovirus (CMV) was present in both the cell and supernatant fractions, including 28 (80%) and 29 (83%), respectively, of 35 BAL samples with corresponding biopsies that showed evidence of CMV pneumonitis and 34 (15%) and 75 (33%), respectively, of 227 BAL samples with corresponding biopsies that did not. Cultures of unseparated BAL fluid had a similar yield to cultures of supernatant. Virus titers of cell fractions and supernatants from BAL samples with corresponding biopsies that were positive for CMV pneumonitis were higher than those from BALs with negative corresponding biopsies. Culture of the unseparated fluid is recommended for simplicity and to maximize detection of CMV. Quantitative results may help in the identification of CMV pneumonitis, but additional studies are needed before quantitative cultures can be recommended for routine use.
对肺移植受者支气管肺泡灌洗（BAL）液的细胞和上清液部分进行定量空斑试验培养的结果，与经支气管肺活检的结果进行了比较。巨细胞病毒（CMV）在细胞和上清液部分均有出现，在35份BAL样本中，分别有28份（80%）和29份（83%）对应的活检显示有CMV肺炎证据，在227份BAL样本中，分别有34份（15%）和75份（33%）对应的活检未显示CMV肺炎证据。未分离的BAL液培养物的产量与上清液培养物相似。CMV肺炎活检呈阳性的BAL样本的细胞部分和上清液的病毒滴度高于相应活检呈阴性的BAL样本。为简便起见并最大限度地检测CMV，建议对未分离的液体进行培养。定量结果可能有助于CMV肺炎的识别，但在推荐将定量培养用于常规使用之前，还需要进行更多研究。
