Gleaves C A, Myerson D, Bowden R A, Hackman R C, Meyers J D
Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington.
J Clin Microbiol. 1989 Nov;27(11):2429-32. doi: 10.1128/jcm.27.11.2429-2432.1989.
An in situ DNA hybridization assay was compared with centrifugation culture for rapid detection of cytomegalovirus (CMV) from bronchoalveolar lavage (BAL) samples. Eighty BAL samples were inoculated into both centrifugation culture and standard culture. Cytospin preparations of the BAL samples were studied in a 75-min in situ DNA hybridization assay using the PathoGene CMV kit (Enzo Biochem, Inc., New York, N.Y.). Of the 80 samples, 39 (49%) were positive for CMV; 37 of 39 (95%) were positive by centrifugation culture, 34 of 39 (87%) were positive in standard culture, 24 of 39 (62%) were positive by in situ hybridization, and 20 of 39 (56%) were positive by histologic and/or immunofluorescence techniques. The in situ hybridization assay detected 23 of the 37 samples positive in centrifugation culture, for a sensitivity of 62% and a specificity of 98%. We conclude that the in situ hybridization assay is a specific and more rapid test than centrifugation culture and standard culture for diagnosis of CMV pulmonary infection. For the clinical laboratory, however, current hybridization methods are not sufficiently sensitive to replace centrifugation culture for detection of CMV in BAL specimens.
将原位DNA杂交检测法与离心培养法进行比较，以从支气管肺泡灌洗（BAL）样本中快速检测巨细胞病毒（CMV）。将80份BAL样本分别接种于离心培养和标准培养。使用PathoGene CMV试剂盒（纽约州纽约市的Enzo Biochem公司），在75分钟的原位DNA杂交检测中研究BAL样本的细胞离心涂片制剂。在这80份样本中，39份（49%）CMV呈阳性；39份中的37份（95%）通过离心培养呈阳性，39份中的34份（87%）在标准培养中呈阳性，39份中的24份（62%）通过原位杂交呈阳性，39份中的20份（56%）通过组织学和/或免疫荧光技术呈阳性。原位杂交检测法在离心培养呈阳性的37份样本中检测出23份，灵敏度为62%，特异性为98%。我们得出结论，对于诊断CMV肺部感染，原位杂交检测法是一种比离心培养和标准培养更特异且更快速的检测方法。然而，对于临床实验室而言，目前的杂交方法在检测BAL标本中的CMV时灵敏度不足以替代离心培养。
