Ishioka C, Yoshida A, Kimata H, Mikawa H
Department of Paediatrics, Faculty of Medicine, Kyoto University Hospital, Japan.
Clin Exp Immunol. 1992 Mar;87(3):504-8. doi: 10.1111/j.1365-2249.1992.tb03027.x.
The effect of vasoactive intestinal peptide (VIP) on human lymphoblastoid B cell lines and tonsil B cells was studied. VIP increased immunoglobulin production and proliferation by lymphoblastoid B cell line, GM-1056, in a dose-dependent manner. As little as 10(-12) M of VIP was effective, and higher concentrations of VIP induced an approximately five-fold increase in IgA production. Moreover, this enhancement was blocked by VIP antagonist. Similarly, VIP enhanced IgM and IgG production by other lymphoblastoid B cell lines, CBL and IM-9, respectively. In contrast to VIP, another neuropeptide substance P (SP) or somatostatin failed to enhance immunoglobulin production and thymidine uptake. VIP also enhanced IgA production and thymidine uptake by purified tonsil B cells. However, in contrast to B cell lines, VIP failed to enhance IgM and IgG production by tonsil B cells. SP or somatostatin failed to enhance immunoglobulin production or thymidine uptake by tonsil B cells. These results indicate that VIP acts as B cell stimulatory factor and that VIP may also have preferential effect on IgA production on tonsil B cells.
研究了血管活性肠肽（VIP）对人淋巴母细胞样B细胞系和扁桃体B细胞的作用。VIP以剂量依赖性方式增加淋巴母细胞样B细胞系GM-1056的免疫球蛋白产生和增殖。低至10^(-12) M的VIP即有效，更高浓度的VIP可使IgA产生增加约五倍。此外，这种增强作用可被VIP拮抗剂阻断。同样，VIP分别增强了其他淋巴母细胞样B细胞系CBL和IM-9的IgM和IgG产生。与VIP相反，另一种神经肽P物质（SP）或生长抑素未能增强免疫球蛋白产生和胸苷摄取。VIP还增强了纯化扁桃体B细胞的IgA产生和胸苷摄取。然而，与B细胞系不同，VIP未能增强扁桃体B细胞的IgM和IgG产生。SP或生长抑素未能增强扁桃体B细胞的免疫球蛋白产生或胸苷摄取。这些结果表明VIP作为B细胞刺激因子，并且VIP可能对扁桃体B细胞的IgA产生具有优先作用。
