胃特异性核蛋白识别的H⁺/K⁺ATP酶α和β亚基基因调控区的序列基序。

Sequence motif in control regions of the H+/K+ ATPase alpha and beta subunit genes recognized by gastric specific nuclear protein(s).

作者信息

Tamura S, Oshiman K, Nishi T, Mori M, Maeda M, Futai M

机构信息

Department of Organic Chemistry and Biochemistry, Osaka University, Japan.

出版信息

FEBS Lett. 1992 Feb 24;298(2-3):137-41. doi: 10.1016/0014-5793(92)80040-n.

DOI:10.1016/0014-5793(92)80040-n

PMID:1312019

Abstract

A nuclear protein(s) from rat or pig stomach recognized a conserved sequence in the 5'-upstream regions of the rat and human H+/K(+)-ATPase alpha subunit genes. A gel retardation assay suggested that part of the binding site was located in the TAATCAGCTG sequence. No nuclear proteins capable of the binding could be detected in other tissues of rat (liver, brain, kidney, spleen and lung) or pig liver. The sequence motif (GATAGC) located 5'-upstream of the beta-subunit gene also seemed to be recognized by the same protein, because the binding of nuclear protein to the sequence motifs in the alpha and beta subunits was mutually competitive. Considering the sense-strand sequence of the binding motif in the alpha-subunit gene, we conclude that (G/C)PuPu(G/C)NGAT(A/T)PuPy is a core sequence motif for the gastric specific DNA binding protein (PCSF, parietal cell specific factor).

摘要

来自大鼠或猪胃的一种核蛋白识别出大鼠和人类H⁺/K⁺-ATP酶α亚基基因5'-上游区域中的一个保守序列。凝胶阻滞试验表明部分结合位点位于TAATCAGCTG序列中。在大鼠的其他组织（肝脏、脑、肾脏、脾脏和肺）或猪肝中未检测到能够结合的核蛋白。位于β亚基基因5'-上游的序列基序（GATAGC）似乎也被同一蛋白识别，因为核蛋白与α和β亚基中的序列基序的结合相互竞争。考虑到α亚基基因中结合基序的有义链序列，我们得出结论(G/C)PuPu(G/C)NGAT(A/T)PuPy是胃特异性DNA结合蛋白（PCSF，壁细胞特异性因子）的核心序列基序。