Nemec Alexandr, Dijkshoorn Lenie, Cleenwerck Ilse, De Baere Thierry, Janssens Danielle, van der Reijden Tanny J K, Ježek Petr, Vaneechoutte Mario
National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
Department of Infectious Diseases, Leiden University Medical Center C5-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1563-1567. doi: 10.1099/ijs.0.02631-0.
The taxonomic status of seven glucose-non-acidifying, non-proteolytic Acinetobacter strains characterized by forming small colonies on agar media was studied. With one exception, all strains were from human specimens. They could be distinguished from all described Acinetobacter (genomic) species by their ability to grow on ethanol and acetate as sole sources of carbon but not on 22 other substrates tested including DL-lactate or DL-4-aminobutyrate. DNA-DNA hybridization studies, 16S rRNA gene sequence analysis, amplified rDNA restriction analysis and DNA polymorphism analysis by AFLP showed that these strains represent a hitherto unknown species of the genus Acinetobacter, for which the name Acinetobacter parvus (type strain LMG 21765(T)=LUH 4616(T)=NIPH 384(T)=CCM 7030(T)) is proposed.
对七株葡萄糖非酸化、非蛋白水解的不动杆菌菌株进行了分类学地位研究，这些菌株的特征是在琼脂培养基上形成小菌落。除一株外，所有菌株均来自人类标本。它们能够以乙醇和乙酸盐作为唯一碳源生长，但不能在包括DL-乳酸或DL-4-氨基丁酸盐在内的其他22种测试底物上生长，以此可与所有已描述的不动杆菌（基因组）物种区分开来。DNA-DNA杂交研究、16S rRNA基因序列分析、扩增rDNA限制性分析以及AFLP法进行的DNA多态性分析表明，这些菌株代表了不动杆菌属中一个迄今未知的物种，为此提出名为Acinetobacter parvus（模式菌株LMG 21765(T)=LUH 4616(T)=NIPH 384(T)=CCM 7030(T)）。
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