Krizova Lenka, McGinnis Jana, Maixnerova Martina, Nemec Matej, Poirel Laurent, Mingle Lisa, Sedo Ondrej, Wolfgang William, Nemec Alexandr
Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, Albany, NY 12201-2002, USA.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):857-863. doi: 10.1099/ijs.0.000028. Epub 2014 Dec 15.
We aimed to define the taxonomic status of 16 strains which were phenetically congruent with Acinetobacter DNA group 15 described by Tjernberg & Ursing in 1989. The strains were isolated from a variety of human and animal specimens in geographically distant places over the last three decades. Taxonomic analysis was based on an Acinetobacter-targeted, genus-wide approach that included the comparative sequence analysis of housekeeping, protein-coding genes, whole-cell profiling based on matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), an array of in-house physiological and metabolic tests, and whole-genome comparative analysis. Based on analyses of the rpoB and gyrB genes, the 16 strains formed respective, strongly supported clusters clearly separated from the other species of the genus Acinetobacter. The distinctness of the group at the species level was indicated by average nucleotide identity values of ≤82 % between the whole genome sequences of two of the 16 strains (NIPH 2171(T) and NIPH 899) and those of the known species. In addition, the coherence of the group was also supported by MALDI-TOF MS. All 16 strains were non-haemolytic and non-gelatinase-producing, grown at 41 °C and utilized a rather limited number of carbon sources. Virtually every strain displayed a unique combination of metabolic and physiological features. We conclude that the 16 strains represent a distinct species of the genus Acinetobacter, for which the name Acinetobacter variabilis sp. nov. is proposed to reflect its marked phenotypic heterogeneity. The type strain is NIPH 2171(T) ( = CIP 110486(T) = CCUG 26390(T) = CCM 8555(T)).
我们旨在确定16株菌株的分类地位，这些菌株在表型上与1989年Tjernberg和Ursing描述的不动杆菌DNA群15一致。这些菌株是在过去三十年中从地理上相距遥远的各种人类和动物标本中分离出来的。分类分析基于一种针对不动杆菌的全属方法，包括看家蛋白编码基因的比较序列分析、基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的全细胞分析、一系列内部生理和代谢测试以及全基因组比较分析。基于rpoB和gyrB基因的分析，这16株菌株形成了各自得到有力支持的聚类，明显与不动杆菌属的其他物种分开。16株菌株中的两株（NIPH 2171(T)和NIPH 899）与已知物种的全基因组序列之间的平均核苷酸同一性值≤82%，表明该类群在物种水平上具有独特性。此外，MALDI-TOF MS也支持该类群的一致性。所有16株菌株均不溶血且不产生明胶酶，能在41°C生长，利用的碳源数量相当有限。几乎每株菌株都表现出独特的代谢和生理特征组合。我们得出结论，这16株菌株代表不动杆菌属的一个独特物种，为此提议将其命名为可变不动杆菌新种（Acinetobacter variabilis sp. nov.），以反映其显著的表型异质性。模式菌株为NIPH 2171(T)（=CIP 110486(T)=CCUG 26390(T)=CCM 8555(T)）。
