Yassin A F, Sträubler B, Schumann P, Schaal K P
Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, D-53127 Bonn, Germany.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1595-1599. doi: 10.1099/ijs.0.02593-0.
A bacterial isolate (IMMIB R-145(T)) from a human abscess was subjected to a polyphasic taxonomic study. Chemotaxonomic investigations revealed the isolate to have cell wall chemotype IV and mycolic acids consistent with characteristics of the genus NOCARDIA: Comparative 16S rRNA gene sequencing showed that the isolate constituted a distinct subline within the genus Nocardia, displaying greater than 1.7 % sequence divergence with established species. However, a DNA-DNA hybridization study demonstrated unambiguously that the isolate was genealogically distinct from closely related species, namely, Nocardia abscessus, Nocardia cyriacigeorgica and Nocardia farcinica, with which it showed high levels of 16S rDNA sequence similarity (97.8, 97.9 and 98.3 %, respectively). Based on phenotypic and phylogenetic data, it is proposed that this isolate be classified as a new species of the genus Nocardia, for which the name Nocardia puris (type strain IMMIB R-145(T)=DSM 44599(T)=NRRL B-24204(T)) is proposed.
对从人类脓肿中分离出的一株细菌（IMMIB R-145(T)）进行了多相分类研究。化学分类学研究表明，该分离株具有IV型细胞壁化学型和与诺卡氏菌属特征相符的分枝菌酸：比较16S rRNA基因测序显示，该分离株在诺卡氏菌属内构成一个独特的亚系，与已确定的物种显示出大于1.7%的序列差异。然而，DNA-DNA杂交研究明确表明，该分离株在谱系上与密切相关的物种，即脓肿诺卡氏菌、乔治亚州诺卡氏菌和豚鼠耳炎诺卡氏菌不同，它与这些物种显示出高水平的16S rDNA序列相似性（分别为97.8%、97.9%和98.3%）。基于表型和系统发育数据，建议将该分离株分类为诺卡氏菌属的一个新物种，为此提议将其命名为诺卡氏菌纯化种（模式菌株IMMIB R-145(T)=DSM 44599(T)=NRRL B-24204(T)）。
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