Christensen M K, Frederickson C J, Danscher G
Department of Neurobiology, University of Aarhus, Denmark.
J Histochem Cytochem. 1992 Apr;40(4):575-9. doi: 10.1177/40.4.1313065.
The autometallographic retrograde tracing of zinc-containing neurons by intracerebral injection of sodium selenite (Na2SeO3), introduced by Danscher in 1982, has recently been described in more detail. Intracerebral injections of both sodium selenide (Na2Se) and sodium selenite (Na2SeO3) have been successfully used; however, sodium selenite had a rather toxic effect on the injected tissue. In the present study, we tested seven different selenium compounds to find the most suitable compound for retrograde tracing of zinc-positive pathways. Among the tested compounds, sodium selenide (Na2Se) caused insignificant necrosis within the injection site and was easily transported retrogradely when handled anaerobically. Sodium selenide is therefore recommended as the compound of choice.
1982年丹舍尔提出的通过脑内注射亚硒酸钠（Na2SeO3）对含锌神经元进行自动放射自显影逆行追踪，近来已有更详细的描述。脑内注射硒化钠（Na2Se）和亚硒酸钠（Na2SeO3）均已成功应用；然而，亚硒酸钠对注射组织有相当大的毒性作用。在本研究中，我们测试了七种不同的硒化合物，以找到最适合用于锌阳性通路逆行追踪的化合物。在所测试的化合物中，硒化钠（Na2Se）在注射部位引起的坏死不明显，并且在厌氧处理时易于逆行运输。因此，推荐使用硒化钠作为首选化合物。
