Slomianka L, Danscher G, Frederickson C J
Department of Neurobiology, University of Aarhus, Denmark.
Neuroscience. 1990;38(3):843-54. doi: 10.1016/0306-4522(90)90076-g.
Intraperitoneal injections of sodium selenite result in the formation of zinc-selenium complexes in zinc-containing axonal boutons ("Timm stainable boutons"), and the zinc-selenium precipitate can be rendered visible in histological sections by silver enhancement. In this work we present evidence, in the rat, that zinc-selenium precipitates formed in vivo after intraperitoneal injections of sodium selenite are translocated by colchicine-sensitive retrograde transport to neural perikarya when animals are allowed to survive 12-24 h after the selenite administration. Silver enhancement renders the perikaryal precipitates visible and thus demonstrates the perikarya of all zinc-containing neurons in the CNS simultaneously. Large populations of zinc-containing neurons identified by the method are found in layers II, III, and VI of all neocortical areas, in the superficial and deep layers of the prepyriform areas and, with a high degree of regional differentiation, in the retrosplenial, entorhinal, para- and presubicular cortices, the hippocampal formation and the amygdaloid complex. Zinc-containing cells were absent from the caudate-putamen, nucleus accumbens and septal complex. Labeled zinc-containing cells are absent in non-telencephalic parts of the brain. The findings indicate that the zinc-containing circuitry of the brain mainly serves in telencephalic information processing.
腹腔注射亚硒酸钠会导致在含锌的轴突终扣（“Timm 染色阳性终扣”）中形成锌 - 硒复合物，并且通过银增强法可使锌 - 硒沉淀物在组织学切片中显现出来。在这项研究中，我们提供了大鼠方面的证据，即腹腔注射亚硒酸钠后在体内形成的锌 - 硒沉淀物，在亚硒酸钠给药后让动物存活 12 - 24 小时时，会通过秋水仙碱敏感的逆行运输转移到神经核周体。银增强法使核周体沉淀物可见，从而同时显示出中枢神经系统中所有含锌神经元的核周体。通过该方法鉴定出的大量含锌神经元存在于所有新皮质区域的 II、III 和 VI 层、梨状前区的浅层和深层，并且在 retrosplenial、内嗅、旁海马和前下托皮质、海马结构和杏仁复合体中具有高度的区域分化。尾状核 - 壳核、伏隔核和隔复合体中不存在含锌细胞。大脑非端脑部分不存在标记的含锌细胞。这些发现表明，大脑中含锌的神经回路主要服务于端脑的信息处理。
