Howell G A, Frederickson C J
Laboratory for Neurobiology, University of Texas at Dallas, Richardson 75083.
Brain Res. 1990 May 7;515(1-2):277-86. doi: 10.1016/0006-8993(90)90607-d.
A method for selectively labeling the CNS neurons that give rise to zinc-containing axonal boutons is described. Focal intracerebral infusions of selenium anions are used to produce a precipitate (presumably ZnSe) in zinc-containing axonal boutons at the injection site, and within 24-48 h, the precipitate is transported to the neurons of origin of those boutons. Silver enhancement is used to render the ZnSe visible in sections prepared for the light or electron microscope. No evidence of retrograde labeling is apparent in neurons that do not contain zinc in their boutons. Thus, the method provides a chemically specific technique for retrograde tracing of zinc-containing pathways in the CNS.
本文描述了一种选择性标记中枢神经系统（CNS）中产生含锌轴突终扣的神经元的方法。通过向脑内局部注入硒阴离子，在注射部位的含锌轴突终扣中产生沉淀（推测为ZnSe），在24 - 48小时内，该沉淀被转运至这些终扣的起源神经元。利用银增强技术使ZnSe在用于光学显微镜或电子显微镜观察的切片中可见。在其终扣中不含锌的神经元中未观察到逆行标记的证据。因此，该方法为中枢神经系统中含锌通路的逆行追踪提供了一种化学特异性技术。
