Mandava P, Howell G A, Frederickson C J
Laboratory for Neurobiology, University of Texas, Dallas, Richardson 75083.
Brain Res. 1993 Apr 9;608(1):115-22. doi: 10.1016/0006-8993(93)90781-h.
A zinc-specific retrograde transport method has been employed to map the zinc-containing neuronal projections to the septal nuclei. Sodium selenite was infused iontophoretically into the lateral or medial septal nuclei to precipitate vesicular zinc as ZnSe in situ, and the neurons that were subsequently labeled by the retrograde transport of ZnSe to their perikarya were mapped. Zinc-containing cells of origin were found only in the hippocampal formation and predominantly in two regions thereof: (i) in s. oriens and deep s. pyramidale of fields CA3a and CA2 and (ii) in s. pyramidale of distal CA1 and adjacent prosubiculum.
一种锌特异性逆行运输方法已被用于绘制含锌神经元向隔核的投射。将亚硒酸钠通过离子电泳注入外侧或内侧隔核，使囊泡锌原位沉淀为硒化锌，并绘制随后通过硒化锌逆行运输至其胞体而被标记的神经元。发现含锌的起源细胞仅存在于海马结构中，且主要存在于其中的两个区域：（i）CA3a和CA2区的海马下托和深层锥体层；（ii）远端CA1区和相邻前下托的锥体层。
