用于检测马疱疹病毒1型和4型DNA的聚合酶链反应（PCR）

[The polymerase chain reaction (PCR) for the detection of DNA of equine herpesviruses 1 and 4].

作者信息

Hardt M, Teifke J P, Weiss E

机构信息

Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen.

出版信息

Berl Munch Tierarztl Wochenschr. 1992 Feb 1;105(2):52-5.

PMID:1313672

Abstract

Formalin-fixed and Paraplast-embedded tissue samples of 42 aborted equine fetuses were examined by polymerase chain reaction for the presence of equine herpesvirus DNA. The used set of primers was located in the glycoprotein 13 open reading frame and allowed the amplification of both EHV 1 und EHV 4. By cleaving pattern analysis after Hinf I digestion EHV 1 could be distinguished from EHV 4. In 9 of the cases investigated EHV 1-DNA was detected. This finding is in absolute context with the results of the virological investigations.

摘要

对42例流产马胎儿的福尔马林固定、石蜡包埋组织样本进行聚合酶链反应检测，以确定是否存在马疱疹病毒DNA。所用引物位于糖蛋白13开放阅读框中，可扩增EHV 1和EHV 4。通过Hinf I消化后的酶切图谱分析，可将EHV 1与EHV 4区分开来。在所调查的9例病例中检测到EHV 1-DNA。这一发现与病毒学调查结果完全相符。