单纯疱疹病毒1型的ICP0而非ICP27与纯化病毒粒子的关联。

Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.

J Virol. 1992 May;66(5):2709-16. doi: 10.1128/JVI.66.5.2709-2716.1992.

Recent studies have shown that ICP4, one of the major immediate-early proteins of herpes simplex virus type 1 is present within the tegument region of the virion (F. Yao and R. J. Courtney, J. Virol. 63:3338-3344, 1989). With monoclonal antibodies to two additional immediate-early proteins, ICP0 and ICP27, and Western blot (immunoblot) analysis, ICP0, but not ICP27, was also found to be associated with purified virus particles. In an effort to localize the ICP0 within the virion, purified virions were treated with trypsin in the presence and absence of detergent. The data suggest that ICP0 is located within the tegument region of the virion and is not localized in the envelope or within the nucleocapsid. The number of molecules of ICP0 per virion was estimated to be approximately 150.

最近的研究表明，单纯疱疹病毒1型的主要立即早期蛋白之一ICP4存在于病毒体的被膜区域（F. Yao和R. J. Courtney，《病毒学杂志》63:3338 - 3344，1989年）。利用针对另外两种立即早期蛋白ICP0和ICP27的单克隆抗体以及蛋白质免疫印迹分析，发现ICP0与纯化的病毒颗粒相关联，而ICP27则不然。为了在病毒体内定位ICP0，在有和没有去污剂存在的情况下，用胰蛋白酶处理纯化的病毒体。数据表明，ICP0位于病毒体的被膜区域，而不在包膜或核衣壳内。每个病毒体中ICP0的分子数估计约为150个。