Knox K K, Carrigan D R
Department of Pathology, Medical College of Wisconsin, Milwaukee 53226.
J Infect Dis. 1992 May;165(5):925-9. doi: 10.1093/infdis/165.5.925.
Suppression of marrow function may be one of the most serious effects of human herpesvirus 6 (HHV-6) infection in marrow transplant patients. In this study, normal bone marrow mononuclear cells were infected in vitro with HHV-6, and a methylcellulose-based colony formation assay was used to evaluate the impact of the infection on marrow cell differentiation and proliferation. Results demonstrated that the outgrowth of colony-forming units of granulocyte and macrophage lineages (cfu-GM) was decreased by approximately 43%, that growth of cfu of granulocyte, erythrocyte, macrophage, and megakaryocyte lineages (cfu-GEMM) was inhibited by an average of 71%, and that the erythroid burst-forming unit (bfu-E) was decreased by approximately 73%. Further, outgrowth of the marrow stromal layer was reduced 74%. Direct infection of bone marrow monocytes was observed, although cell-free virus could not be detected in infected culture supernatants. Addition of a neutralizing monoclonal antibody specific for interferon-alpha to the infected cultures resulted in an almost complete reversal of the viral suppressive effects.
骨髓功能抑制可能是人类疱疹病毒6型（HHV - 6）感染骨髓移植患者最严重的影响之一。在本研究中，正常骨髓单个核细胞在体外被HHV - 6感染，并用基于甲基纤维素的集落形成试验来评估感染对骨髓细胞分化和增殖的影响。结果表明，粒细胞和巨噬细胞谱系集落形成单位（cfu - GM）的生长减少了约43%，粒细胞、红细胞、巨噬细胞和巨核细胞谱系集落形成单位（cfu - GEMM）的生长平均受到71%的抑制，红系爆式集落形成单位（bfu - E）减少了约73%。此外，骨髓基质层的生长减少了74%。观察到骨髓单核细胞的直接感染，尽管在感染的培养上清液中未检测到游离病毒。向感染培养物中添加针对α干扰素的中和单克隆抗体几乎完全逆转了病毒的抑制作用。
