Isomura H, Yamada M, Yoshida M, Tanaka H, Kitamura T, Oda M, Nii S, Seino Y
Department of Pediatrics, Okayama University Medical School, Shikatacho, Japan.
J Med Virol. 1997 Aug;52(4):406-12. doi: 10.1002/(sici)1096-9071(199708)52:4<406::aid-jmv11>3.0.co;2-e.
Human herpesvirus 6 (HHV-6) has been reported to be involved in bone marrow failure after bone marrow transplantation (BMT). To elucidate the role of HHV-6 in the marrow failure, we examined the comparative effect of two variants of HHV-6 (HHV-6A and HHV-6B) and human herpesvirus 7 (HHV-7) on in vitro colony formation of hematopoietic progenitor cells in methylcellulose semi-solid media. Progenitor cells prepared from cord blood mononuclear cells (CBMNCs) were infected with one of these viruses at various multiplicity of infection (MOI), and were subjected to methylcellulose colony assay. Formation of both granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) colonies was MOI-dependently suppressed after infection with the Z29 strain of HHV-6B. Although HHV-6A suppressed the formation of BFU-E colonies as efficiently as HHV-6B, the former did not exhibit significant suppressive effect on the formation of CFU-GM colonies at an MOI 1. HHV-7 had no effect on hematopoietic colony formation at all. Based on frequent positivity of viral DNA in single colonies obtained from HHV-6-infected progenitor cells by polymerase chain reaction and in situ hybridization, direct effects of HHV-6 on the hematopoietic progenitor cells are suggested as the cause of the suppression rather than indirect effects via accessory cells of the bone marrow.
据报道，人类疱疹病毒6型（HHV-6）与骨髓移植（BMT）后的骨髓衰竭有关。为了阐明HHV-6在骨髓衰竭中的作用，我们在甲基纤维素半固体培养基中检测了HHV-6的两种变体（HHV-6A和HHV-6B）以及人类疱疹病毒7型（HHV-7）对造血祖细胞体外集落形成的比较影响。从脐血单个核细胞（CBMNCs）制备的祖细胞以不同的感染复数（MOI）感染这些病毒之一，然后进行甲基纤维素集落测定。用HHV-6B的Z29株感染后，粒细胞/巨噬细胞（CFU-GM）集落和红系（BFU-E）集落的形成均呈MOI依赖性抑制。尽管HHV-6A抑制BFU-E集落形成的效率与HHV-6B相同，但在MOI为1时，前者对CFU-GM集落的形成未表现出显著的抑制作用。HHV-7对造血集落形成完全没有影响。基于通过聚合酶链反应和原位杂交从感染HHV-6的祖细胞获得的单个集落中病毒DNA的频繁阳性，提示HHV-6对造血祖细胞的直接作用是抑制的原因，而不是通过骨髓辅助细胞的间接作用。
