In vitro transcription and translation of genomic RNA from a porcine group C rotavirus.

Eleven segments of ssRNA were synthesized from dsRNAs of a porcine group (Gp) C rotavirus (Cowden strain) using an in vitro transcription system. In vitro translation of unfractionated ssRNAs revealed at least nine viral proteins, ranging from 22 kDa to 93 kDa. The 37 kDa and 25 kDa proteins were glycosylated as demonstrated by the endoglycosidase H assay. In vitro translated products analyzed by SDS-polyacrylamide gel electrophoresis and partial protease peptide mapping were comparable to those synthesized in vivo (MA 104 cells).