Jiang B M, Saif L J
Food Animal Health Research Program, Ohio State University, Wooster.
Arch Virol. 1992;124(1-2):181-5. doi: 10.1007/BF01314636.
Eleven segments of ssRNA were synthesized from dsRNAs of a porcine group (Gp) C rotavirus (Cowden strain) using an in vitro transcription system. In vitro translation of unfractionated ssRNAs revealed at least nine viral proteins, ranging from 22 kDa to 93 kDa. The 37 kDa and 25 kDa proteins were glycosylated as demonstrated by the endoglycosidase H assay. In vitro translated products analyzed by SDS-polyacrylamide gel electrophoresis and partial protease peptide mapping were comparable to those synthesized in vivo (MA 104 cells).
使用体外转录系统从猪C组轮状病毒（考登毒株）的双链RNA合成了11条单链RNA片段。未分级单链RNA的体外翻译显示至少有9种病毒蛋白，分子量范围为22 kDa至93 kDa。内切糖苷酶H分析表明，37 kDa和25 kDa的蛋白被糖基化。通过SDS-聚丙烯酰胺凝胶电泳和部分蛋白酶肽图谱分析的体外翻译产物与体内（MA 104细胞）合成的产物相当。
