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猪C组轮状病毒（副轮状病毒）在原代猪肾细胞培养物中的连续传代

Serial propagation of porcine group C rotavirus (pararotavirus) in primary porcine kidney cell cultures.

作者信息

Terrett L A, Saif L J

出版信息

J Clin Microbiol. 1987 Jul;25(7):1316-9. doi: 10.1128/jcm.25.7.1316-1319.1987.

DOI:10.1128/jcm.25.7.1316-1319.1987
PMID:3038949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269204/
Abstract

A porcine group C rotavirus was adapted to serial propagation in roller tube cultures of primary porcine kidney cells with high concentrations of pancreatin. Infected cells were identified by immunofluorescence staining of cell monolayers. Only group C rotavirus particles were observed in culture supernatants by immune electron microscopy.

摘要

一株猪C组轮状病毒在含有高浓度胰蛋白酶的原代猪肾细胞滚瓶培养物中连续传代适应。通过细胞单层的免疫荧光染色鉴定感染细胞。通过免疫电子显微镜在培养上清液中仅观察到C组轮状病毒颗粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/c8fefe6e2ea5/jcm00091-0196-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/25fdf807a03c/jcm00091-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/e0ed2d7edb33/jcm00091-0195-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/9cbde52d745e/jcm00091-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/c8fefe6e2ea5/jcm00091-0196-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/25fdf807a03c/jcm00091-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/e0ed2d7edb33/jcm00091-0195-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/9cbde52d745e/jcm00091-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7c1/269204/c8fefe6e2ea5/jcm00091-0196-b.jpg

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Characterization of a human pararotavirus.一种人类副轮状病毒的特性描述。
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Sequence comparison of the VP7 gene encoding the outer capsid glycoprotein among animal and human group C rotaviruses.动物和人类C组轮状病毒中编码外衣壳糖蛋白的VP7基因的序列比较。
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