Mori S, Ohkoshi S, Hijikata M, Kato N, Shimotohno K
Virology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1992 Mar;83(3):264-8. doi: 10.1111/j.1349-7006.1992.tb00098.x.
Infection with hepatitis C virus (HCV) was analyzed by an enzyme-linked immunosorbent assay based on recombinant viral proteins encoded by regions of the putative viral core, NS3, NS4 and NS5, which were expressed in E. coli. Results showed that 106 of 124 cases (85.5%) of non-A, non-B chronic hepatitis and 43 of 45 cases (95.5%) of hepatocellular carcinoma, negative for HBV marker, were positive for antibodies against at least one of these viral proteins. One of 87 healthy individuals with normal alanine aminotransferase activity was positive for antibody against only the viral core, but was negative for HCV RNA. The serum of one patient with chronic hepatitis was positive for one of these proteins, but negative for HCV RNA. These findings in combination with results on detection of HCV RNA in the sera of patients with non-A, non-B chronic hepatitis indicated that 105 of 124 cases (84.6%) were positive for HCV infection. Sera that were negative for HCV antibodies against all these proteins were also negative for HCV RNA assayed by reverse transcription followed by the polymerase chain reaction. Screening of HCV infection by detecting viral antibodies in circulating blood using all these viral proteins is useful for reducing the number of ambiguous results in screening for viral infection. Thus, this assay system may be useful diagnostic purposes.
采用基于在大肠杆菌中表达的假定病毒核心区、NS3、NS4和NS5区编码的重组病毒蛋白的酶联免疫吸附试验，对丙型肝炎病毒（HCV）感染情况进行分析。结果显示，124例非甲非乙型慢性肝炎患者中有106例（85.5%），45例HBV标志物阴性的肝细胞癌患者中有43例（95.5%），针对这些病毒蛋白中至少一种的抗体呈阳性。87例丙氨酸转氨酶活性正常的健康个体中有1例仅针对病毒核心的抗体呈阳性，但HCV RNA检测为阴性。1例慢性肝炎患者的血清中这些蛋白中的一种呈阳性，但HCV RNA检测为阴性。这些发现与非甲非乙型慢性肝炎患者血清中HCV RNA的检测结果相结合，表明124例患者中有105例（84.6%）HCV感染呈阳性。针对所有这些蛋白的HCV抗体检测为阴性的血清，经逆转录后进行聚合酶链反应检测HCV RNA也为阴性。使用所有这些病毒蛋白通过检测循环血液中的病毒抗体来筛查HCV感染，有助于减少病毒感染筛查中不明确结果的数量。因此，该检测系统可能具有诊断价值。
