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Jpn J Cancer Res. 1991 Aug;82(8):879-82. doi: 10.1111/j.1349-7006.1991.tb01914.x.
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Serodiagnostic assay of hepatitis C virus infection using viral proteins expressed in Escherichia coli.使用在大肠杆菌中表达的病毒蛋白进行丙型肝炎病毒感染的血清学诊断检测。
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[Serodiagnosis of hepatitis C by a recombinant hepatitis C virus protein].
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Synthetic gene for the hepatitis C virus nucleocapsid protein.丙型肝炎病毒核衣壳蛋白的合成基因。
Nucleic Acids Res. 1993 Jun 11;21(11):2747-54. doi: 10.1093/nar/21.11.2747.
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Antigenic regions within the hepatitis C virus envelope 1 and non-structural proteins: identification of an IgG3-restricted recognition site with the envelope 1 protein.丙型肝炎病毒包膜1蛋白和非结构蛋白内的抗原区域：包膜1蛋白上IgG3限制识别位点的鉴定。
Clin Exp Immunol. 1993 Mar;91(3):489-94. doi: 10.1111/j.1365-2249.1993.tb05929.x.
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Serodiagnostic assay of hepatitis C virus infection using viral proteins expressed in Escherichia coli.使用在大肠杆菌中表达的病毒蛋白进行丙型肝炎病毒感染的血清学诊断检测。
Jpn J Cancer Res. 1992 Mar;83(3):264-8. doi: 10.1111/j.1349-7006.1992.tb00098.x.

本文引用的文献

1
Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.从经血传播的非甲非乙型病毒性肝炎基因组中分离出一个cDNA克隆。
Science. 1989 Apr 21;244(4902):359-62. doi: 10.1126/science.2523562.
2
Treatment of chronic hepatitis C with recombinant interferon alfa. A multicenter randomized, controlled trial.重组干扰素α治疗慢性丙型肝炎。一项多中心随机对照试验。
N Engl J Med. 1989 Nov 30;321(22):1501-6. doi: 10.1056/NEJM198911303212203.
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An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.一种针对人类非甲非乙型肝炎主要致病病毒的循环抗体检测方法。
Science. 1989 Apr 21;244(4902):362-4. doi: 10.1126/science.2496467.
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Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.从日本非甲非乙型肝炎患者中克隆人丙型肝炎病毒基因组
Proc Natl Acad Sci U S A. 1990 Dec;87(24):9524-8. doi: 10.1073/pnas.87.24.9524.
5
Detection of hepatitis C virus RNA in sera and liver tissues of non-A, non-B hepatitis patients using the polymerase chain reaction.运用聚合酶链反应检测非甲非乙型肝炎患者血清和肝组织中的丙型肝炎病毒RNA
Jpn J Cancer Res. 1990 Sep;81(9):862-5. doi: 10.1111/j.1349-7006.1990.tb02658.x.
6
Non-specificity of anti-HCV test for seroepidemiological analysis.
Lancet. 1990 Sep 22;336(8717):750-1. doi: 10.1016/0140-6736(90)92245-d.
7
Hepatitis C virus antibodies in chronic active hepatitis: pathogenetic factor or false-positive result?慢性活动性肝炎中的丙型肝炎病毒抗体：致病因素还是假阳性结果？
Lancet. 1990 Mar 31;335(8692):754-7. doi: 10.1016/0140-6736(90)90870-b.
8
A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus.在大肠杆菌中表达的丙型肝炎病毒结构蛋白有助于丙型肝炎病毒的准确检测。
Biochem Biophys Res Commun. 1990 Oct 30;172(2):511-6. doi: 10.1016/0006-291x(90)90702-o.

使用在大肠杆菌中表达的核心蛋白的酶联免疫吸附测定系统检测丙型肝炎病毒感染

Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli.

作者信息

Muraiso K, Hijikata M, Kato N, Shimotohno K, Okazaki N, Ohkoshi S, Uura M, Kaneko S, Kobayashi K, Omata M

机构信息

Virology Division, National Cancer Center Research Institute, Tokyo.

出版信息

Jpn J Cancer Res. 1991 Aug;82(8):879-82. doi: 10.1111/j.1349-7006.1991.tb01914.x.

DOI:10.1111/j.1349-7006.1991.tb01914.x
PMID:1716622
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5918576/
Abstract

Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

摘要

对在大肠杆菌中表达的丙型肝炎病毒（HCV）核心蛋白进行酶联免疫吸附测定，从而检测出慢性肝炎患者体内针对该病毒的抗体。使用不同病毒蛋白检测得到的一些阴性结果，在用这种大肠杆菌表达的病毒蛋白检测时变为阳性，并且病毒RNA检测也呈阳性。因此，使用HCV核心蛋白有助于准确检测HCV感染。