使用在大肠杆菌中表达的核心蛋白的酶联免疫吸附测定系统检测丙型肝炎病毒感染
Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli.
作者信息
Muraiso K, Hijikata M, Kato N, Shimotohno K, Okazaki N, Ohkoshi S, Uura M, Kaneko S, Kobayashi K, Omata M
机构信息
Virology Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1991 Aug;82(8):879-82. doi: 10.1111/j.1349-7006.1991.tb01914.x.
Abstract
Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.
摘要
对在大肠杆菌中表达的丙型肝炎病毒（HCV）核心蛋白进行酶联免疫吸附测定，从而检测出慢性肝炎患者体内针对该病毒的抗体。使用不同病毒蛋白检测得到的一些阴性结果，在用这种大肠杆菌表达的病毒蛋白检测时变为阳性，并且病毒RNA检测也呈阳性。因此，使用HCV核心蛋白有助于准确检测HCV感染。
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