Nickerson K W, Aspedon A
School of Biological Sciences, University of Nebraska, Lincoln 68588-0343.
Mol Microbiol. 1992 Apr;6(8):957-61. doi: 10.1111/j.1365-2958.1992.tb02161.x.
Our work on bacterial detergent resistance started with the realization that bacteria growing in a sink full of soap must be resistant to the detergents in that soap. We chose sodium dodecyl sulphate (SDS) as a model detergent and decided to see how much SDS the bacterium growing in the sink could tolerate. The research program thus initiated has shown that bacteria such as Enterobacter cloacae can grow in up to 25% SDS and that SDS-shock proteins constitute c. 8% of the proteins synthesized by SDS-grown Escherichia coli. It has also provided explanations why enteric bacteria are oxidase negative, and how pyrroloquinoline quinone (PQQ) enters the periplasmic space. Finally, for E. coli, it has provided evidence for an alternate, phosphate-limited, aquatic life style which places greater emphasis on the Entner-Doudoroff pathway. Detergent resistance is important both medically and ecologically, e.g. entry of pathogens via bile-salt-containing intestinal tracts and biodegradation of detergent-like pollutants such as those resulting from oil spills. Our current research is focused on SDS-induced modifications of the cytoplasmic membrane and the presence of SDS in the periplasm.
在装满肥皂的水槽中生长的细菌必定对该肥皂中的洗涤剂具有抗性。我们选择十二烷基硫酸钠（SDS）作为模型洗涤剂，并决定观察水槽中生长的细菌能够耐受多少SDS。由此启动的研究项目表明，阴沟肠杆菌等细菌能够在高达25%的SDS中生长，且SDS应激蛋白约占在SDS中生长的大肠杆菌合成蛋白的8%。该研究还解释了肠道细菌为何氧化酶呈阴性，以及吡咯喹啉醌（PQQ）是如何进入周质空间的。最后，对于大肠杆菌，该研究提供了证据，证明其存在一种更强调Entner-Doudoroff途径的、以磷酸盐限制为特征的水生替代生活方式。抗洗涤剂能力在医学和生态学方面都很重要，例如病原体通过含胆盐的肠道进入以及对类似洗涤剂的污染物（如石油泄漏产生的污染物）进行生物降解。我们目前的研究重点是SDS对细胞质膜的诱导修饰以及周质中SDS的存在情况。
