Harrington L, Wall L V, Kelly D C
Chemical and Biological Defence Establishment, Porton Down, Salisbury, U.K.
J Gen Virol. 1992 May;73 ( Pt 5):1217-26. doi: 10.1099/0022-1317-73-5-1217.
The molecular structure of the genome of simian herpes B virus (SHBV) was determined by restriction endonuclease mapping studies. Genomic DNA was cleaved with restriction endonucleases BamHI and SalI into 41 and 58 fragments, respectively. Most of these fragments were cloned into the plasmid vector pACYC184; uncloned fragments were identified following isolation from agarose gels. Terminal fragments were identified by exonuclease digestion and radioactive end-labelling, and linkage of fragments was deduced by a combination of single and double digest experiments and cross-blot hybridizations. The genome is larger than that of herpes simplex virus type 1 (HSV-1), being approximately 165 kilobase pairs. Like that of HSV-1, the SHBV genome is composed of a long and a short unique region each flanked by inverted repeat sequences, which allow the unique regions to invert relative to one another, resulting in four possible isomeric arrangements of the molecule. Genome locations of several SHBV genes were compared with their HSV-1 homologues.
通过限制性内切酶图谱研究确定了猴B型疱疹病毒（SHBV）基因组的分子结构。基因组DNA分别用限制性内切酶BamHI和SalI切割成41个和58个片段。这些片段大多被克隆到质粒载体pACYC184中；未克隆的片段从琼脂糖凝胶中分离后进行鉴定。通过核酸外切酶消化和放射性末端标记鉴定末端片段，并通过单酶切和双酶切实验及交叉印迹杂交相结合的方法推断片段的连接情况。该基因组比单纯疱疹病毒1型（HSV-1）的基因组大，约为165千碱基对。与HSV-1一样，SHBV基因组由一个长的和一个短的独特区域组成，每个区域两侧都有反向重复序列，这使得独特区域能够相对彼此反转，从而产生该分子的四种可能的异构体排列。将几个SHBV基因的基因组定位与其HSV-1同源物进行了比较。
