粗糙脉孢菌核外突变体的筛选。
Selection of extranuclear mutants of Neurospora crassa.
作者信息
Rosenberg E, Mora C, Edwards D L
出版信息
Genetics. 1976 May;83(1):11-24. doi: 10.1093/genetics/83.1.11.
Abstract
A procedure is described that produce primarily extranuclear mutants, of Neurospora carassa. An analysis of five mutants. [cni-3], [rsp-2], [rsp-3], E1RSP-4], IS PRESENTED. All five mutants segregate in an extranuclear manner. They can be assorted into two classes based on their respiratory properties: (1) those with cyanide-insensitive respiration (cni); (2) those with slow respiration (rsp). All of the mutants are female sterile. The respiratory trait can be placed in different nuclear backgrounds by heterokarytoic transfer. The abnormal respiratory traits are observed in mitochondria isloated from the mutants and it is likely that the mutations are in mitochondrial DNA.
摘要
描述了一种主要产生粗糙脉孢菌核外突变体的程序。对五个突变体[cni-3]、[rsp-2]、[rsp-3]、[rsp-4]进行了分析。所有五个突变体均以核外方式分离。根据其呼吸特性，它们可分为两类：(1) 具有氰化物不敏感呼吸的突变体（cni）；(2) 具有缓慢呼吸的突变体（rsp）。所有突变体均为雌性不育。通过异核体转移，可将呼吸性状置于不同的核背景中。在从突变体中分离出的线粒体中观察到异常呼吸性状，并且这些突变很可能存在于线粒体DNA中。
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