Yamamoto S, Dunn C J, Willoughby D A
Immunology. 1976 Apr;30(4):505-11.
Leucocyte migration inhibitory (MI) activity was investigated in vitro in the delayed hypersensitivity reaction induced by intrapleural injection of PPD into FCA-sensitized guinea-pigs. During the initial reaction (6 h) two types of antigen-dependent MI activity were detected in serum and cell-free exudate. One was of high molecular weight and associated with immunoglobulin and the other was of low molecular weight and appeared to be related to so-called "antigen-dependent MIF". As the reaction progressed (i.e. 12-24 h), two types of antigen-dependent MI activity were revealed in exudate, but not in serum. One was high molecular weight and the other was low molecular weight and thought to be related to so-called "antigen-independent MIF". Similar experiments were performed on the reverse passive Arthus reaction in the pleural cavity of guinea-pigs. A high molecular weight MI activity was detected in 6-h cell-free exudate and was found to be antigen-independent. "So-called MIF" was not found in this reaction.
在将结核菌素纯蛋白衍生物（PPD）经胸膜腔内注射到弗氏完全佐剂致敏的豚鼠所诱导的迟发型超敏反应中，对白细胞迁移抑制（MI）活性进行了体外研究。在初始反应阶段（6小时），在血清和无细胞渗出液中检测到两种抗原依赖性MI活性。一种是高分子量的，与免疫球蛋白相关，另一种是低分子量的，似乎与所谓的“抗原依赖性巨噬细胞移动抑制因子（MIF）”有关。随着反应的进展（即12 - 24小时），在渗出液中发现了两种抗原依赖性MI活性，但在血清中未发现。一种是高分子量的，另一种是低分子量的，被认为与所谓的“抗原非依赖性MIF”有关。在豚鼠胸腔内的反向被动阿图斯反应中进行了类似的实验。在6小时的无细胞渗出液中检测到一种高分子量的MI活性，并且发现其与抗原无关。在该反应中未发现“所谓的MIF”。
